Catalog No: OKEH06855
Size:96T
Price: $525.00
SKU
OKEH06855
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Apcs ELISA Kit (Rat) (OKEH06855) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Apcs has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Apcs is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Apcs captured in the well.
ELISA Kit Range6.25-400ng/mL
ELISA Kit RecoveryMean recovery when spiking into matrix = 102%
ELISA Kit ReproducibilityIntra-assay reproducibility was evaluated with 20 replicates of 3 samples representing low, middle and high level LIPC. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level LIPC using 8 replicates on each of 3 plates.
Intra-Assay CV <5.2%
Inter-Assay CV <9.7%
ELISA Kit Component
ComponentAmount
Apcs Microplate96 Wells (12 x 8 Well strips)
Apcs Lyophilized Standard2 x 400 ng
100X Apcs HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity3.175 ng/mL
SpecificityNatural and recombinant Rat Serum amyloid P-component
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolApcs
Gene Full Nameamyloid P component, serum
Alias SymbolsSap;serum amyloid P-component.
NCBI Gene Id29339
Protein NameP23680
Description of TargetSerum amyloid P-component
Uniprot IDhttps://www.uniprot.org/uniprot/P23680
Protein Accession #NP_058866
Nucleotide Accession #NM_017170
Write Your Own Review
You're reviewing:APCS ELISA Kit (Rat) (OKEH06855)
Your Rating
We found other products you might like!