Apcs ELISA Kit (Mouse) (OKBB01530)

Catalog No: OKBB01530

Size:96 wells

Apcs ELISA Kit (Mouse) (OKBB01530) Standard Curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva's mouse SAP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for SAP has been precoated onto 96-well plates. Standards(NS0, Q21-D224) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SAP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse SAP amount of sample captured in plate.
• ELISA Kit Range: 1.56 ng/ml - 100 ng/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean(pg/ml)	3608	12778	47613	3726	13449	43062
  Standard deviation	144.32	715.56	2809.16	176.12	981.77	2626.78
  CV(%)	4	5.6	5.9	4.7	7.3	6.1

• ELISA Kit Component:
  • 96-Well Plate Pre-coated with Anti-Mouse Apcs Antibody: 96 Wells (12 x 8 Well Strips)
  • Lyophilized Recombinant Mouse Apcs Standard: 2 x 10 ng
  • 100X Biotinylated Anti-Mouse Apcs Antibody: 1 x 130 uL
  • 100X Avidin-Biotin Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Additional Information:

  To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

  Lots	Lot1 (pg/ml)	Lot2 (pg/ml)	Lot3 (pg/ml)	Lot4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	3608	4012	4067	4138	3956	205.96	5.2%
  Sample 2	12778	11342	12753	12065	12234	589.38	4.8%
  Sample 3	47613	50001	47706	49348	48667	1034.13	2.1%

  *number of samples for each test n=16.

  
  

  Standard Number	8	7	6	5	4	3	2	1
  Concentration (ng/ml)	0	1.56	3.12	6.25	12.5	25	50	100
  O.D.	0.002	0.127	0.246	0.436	0.787	1.246	1.831	2.328

• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: cell culture supernates,cell lysates, serum and plasma (heparin, EDTA)
• Sensitivity: <50 pg/ml
• Specificity: Natural and recombinant mouse SAP
• Immunogen: Expression system for standard: NS0; Immunogen sequence: Q21-D224
• Description: The Quick version of Picokine ELISA kits, assay takes less than 1.5 hours. Detect Mouse Sap/APCS with <50pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and quick experiment set up.

Target Info

• Gene Symbol: APCS
• Gene Full Name: Serum amyloid P-component
• Alias Symbols: Serum amyloid P-component;SAP;Apcs;Ptx2, Sap;
• NCBI Gene Id: 20219
• Protein Name: Serum amyloid P-component
• Description of Target: Amyloid P component, serum(SAP), also known as PTX2 or APCS, is the identical serum form of amyloid P component(AP), a 25kDa pentameric protein first identified as the pentagonal constituent of in vivo pathological deposits called "amyloid". It belongs to the pentraxins family, characterised by calcium dependent ligand binding and distinctive flattened beta-jellyroll structure similar to that of the legume lectins. This gene is mapped to 1q23.2. The binding of the encoded protein to proteins in the pathological amyloid cross-beta fold suggests its possible role as a chaperone. This protein is also thought to control the degradation of chromatin. It has been demonstrated that this protein binds to apoptotic cells at an early stage, which raises the possibility that it is involved in dealing with apoptotic cells in vivo.
• Uniprot ID: P12246
• Protein Accession #: NP_035448.2
• Nucleotide Accession #: NM_011318.2