APCS ELISA Kit (Human) (OKBB01083)

Catalog No: OKBB01083

Size:96 wells

APCS ELISA Kit (Human) (OKBB01083) Standard Curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva's human SAP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for SAP has been precoated onto 96-well plates. Standards (Expression system for standard: E.coli, Immunogen sequence: H20-V223) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SAP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human SAP amount of sample captured in plate.
• ELISA Kit Range: 1.56 ng/ml - 100 ng/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean(pg/ml)	2959	13066	40903	3227	13910	44719
  Standard deviation	189.37	548.77	2249.66	209.75	737.23	2906.73
  CV(%)	6.4	4.2	5.5	6.5	5.3	6.5

• ELISA Kit Component:
  • 96-Well Plate Pre-coated with Anti-Human APCS Antibody: 96 Wells (12 x 8 Well Strips)
  • Lyophilized Recombinant Human APCS Standard: 2 x 10 ng
  • 100X Biotinylated Anti-Human APCS Antibody: 1 x 130 uL
  • 100X Avidin-Biotin Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Additional Information:

  To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

  Lots	Lot1 (pg/ml)	Lot2 (pg/ml)	Lot3 (pg/ml)	Lot4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	2959	2863	2697	2962	2870	107.66	3.7%
  Sample 2	13066	13637	15280	15579	14390	1063.69	7.3%
  Sample 3	40903	43039	47063	46975	44495	2634.74	5.9%

  *number of samples for each test n=16.

  
  

  Standard Number	8	7	6	5	4	3	2	1
  Concentration (ng/ml)	0	1.56	3.12	6.25	12.5	25	50	100
  O.D.	0.049	0.127	0.204	0.378	0.657	1.038	1.587	1.963

• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: cell culture supernates,cell lysates, serum and plasma (heparin, EDTA)
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant human SAP
• Immunogen: Expression system for standard: E.coli; Immunogen sequence: H20-V223
• Description: For quantitative detection of human SAP in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: APCS
• Gene Full Name: amyloid P component, serum
• Alias Symbols: 9.5S alpha 1 glycoprotein, 9.5S alpha-1-glycoprotein, APCS, P02743, PTX2, Ptx2, Sap, SAMP, SAP, Serum Amyloid P, Serum amyloid Pcomponent, Serum amyloid P-component, Serum amyloid P-component(1-203)
• NCBI Gene Id: 325
• Protein Name: Serum amyloid P-component
• Description of Target: Amyloid P component, serum (SAP), also known as PTX2 or APCS, is the identical serum form of amyloid P component (AP), a 25kDa pentameric protein first identified as the pentagonal constituent of in vivo pathological deposits called "amyloid". It belongs to the pentraxins family, characterised by calcium dependent ligand binding and distinctive flattened β-jellyroll structure similar to that of the legume lectins. This gene is mapped to 1q23.2. The binding of the encoded protein to proteins in the pathological amyloid cross-beta fold suggests its possible role as a chaperone. This protein is also thought to control the degradation of chromatin. It has been demonstrated that this protein binds to apoptotic cells at an early stage, which raises the possibility that it is involved in dealing with apoptotic cells in vivo.
• Uniprot ID: P02743
• Protein Accession #: NP_001630.1
• Nucleotide Accession #: NM_001639.3