APCS Chemi-Luminescent ELISA Kit (Human) (OKCD03910)

Catalog No: OKCD03910

Size:96WELLS

Human APCS ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	80-101%	83-104%	81-98%	91-101%
  EDTA Plasma (n=5)	95-102%	85-92%	78-101%	86-101%
  Heparin Plasma (n=5)	94-103%	78-99%	83-93%	85-99%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Serum Amyloid P Component (SAP). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Serum Amyloid P Component (SAP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Serum Amyloid P Component (SAP) level in the sample or standard.
• ELISA Kit Range: 0.55-400ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	79-96	82
  EDTA Plasma (n=5)	89-101	95
  Heparin Plasma (n=5)	87-104	92

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Serum Amyloid P Component (SAP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Serum Amyloid P Component (SAP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • APCS Microplate: 96 Wells (12 x 8 Well strips)
  • APCS Lyophilized Standard: 2
  • 100X APCS Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 6.25 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: APCS
• Gene Full Name: amyloid P component, serum
• Alias Symbols: 9.5S alpha-1-glycoprotein;epididymis secretory sperm binding protein Li 92n;HEL-S-92n;pentaxin-related;pentraxin-2;pentraxin-related;PTX2;SAP;serum amyloid P-component.
• NCBI Gene Id: 325
• Protein Name: Serum amyloid P-component
• Description of Target: Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. May also function as a calcium-dependent lectin.
• Uniprot ID: P02743
• Protein Accession #: NP_001630
• Nucleotide Accession #: NM_001639