APC ELISA Kit (Rat) (OKCD02308)

Catalog No: OKCD02308

Size:96WELLS

Rat APC ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  sodium citrate plasma(n=5)	84-105%	94-103%	79-99%	78-97%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Activated Protein C (APC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Activated Protein C (APC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Activated Protein C (APC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Activated Protein C (APC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  sodium citrate plasma(n=5)	89-99	95

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Activated Protein C (APC) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Activated Protein C (APC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-APC Microplate: 96 Wells (12 x 8 Well strips)
  • APC Lyophilized Standard: 2
  • 100X Biotinylated APC Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Plasma
• Sensitivity: 0.054 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Activated Protein C (APC). No significant cross-reactivity or interference between Activated Protein C (APC) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Proc
• Gene Full Name: protein C, inactivator of coagulation factors Va and VIIIa
• Alias Symbols: anticoagulant protein C;autoprothrombin IIA;blood coagulation factor XIV;vitamin K-dependent protein C.
• NCBI Gene Id: 25268
• Protein Name: Vitamin K-dependent protein C
• Description of Target: Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids. Exerts a protective effect on the endothelial cell barrier function.
• Uniprot ID: P31394
• Protein Accession #: NP_036935.1
• Nucleotide Accession #: NM_012803.1
• Protein Size (# AA): 461