APC Antibody (OAAF01734)

Catalog No: OAAF01734 (Formerly GWB-ASD009)

Size:100 ug

Western blot analysis of extracts from COLO205 cells, using APC Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using APC Antibody. The picture on the right is blocked with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid. Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human APC.
• Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen
• Concentration: 1mg/ml
• Specificity: APC antibody detects endogenous levels of total APC protein.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1:1000
  IHC: 1:50~1:100
  ELISA: 1:40000
• Reference: T Maltzman, Carcinogenesis, Dec 1997; 18: 2435 - 2439.
  Dhaval M. Patel, J. Immunol., Nov 1999; 163: 5201.
  Qi Sun, J. Immunol., Oct 2000; 165: 4105
  M Matsui, J. Immunol., Jul 1987; 139: 373.

Target Info

• Gene Symbol: APC
• Gene Full Name: APC regulator of WNT signaling pathway
• Alias Symbols: adenomatosis polyposis coli tumor suppressor;adenomatous polyposis coli (APC);adenomatous polyposis coli protein;APC, WNT signaling pathway regulator;BTPS2;deleted in polyposis 2.5;DESMD;DP2;DP2.5;DP3;epididymis secretory sperm binding protein;GS;PPP1R46;protein phosphatase 1, regulatory subunit 46;truncated adenomatosis polyposis coli;WNT signaling pathway regulator.
• NCBI Gene Id: 324
• Protein Name: Adenomatous polyposis coli protein
• Description of Target: Tumor suppressor. Promotes rapid degradation of CTNNB1 and participates in Wnt signaling as a negative regulator. APC activity is correlated with its phosphorylation state. Activates the GEF activity of SPATA13 and ARHGEF4. Plays a role in hepatocyte growth factor (HGF)-induced cell migration. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. It is required for the localization of MACF1 to the cell membrane and this localization of MACF1 is critical for its function in microtubule stabilization.
• Uniprot ID: P25054
• Protein Accession #: NP_000029.2
• Molecular Weight: 311 kDa