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Datasheets/Manuals | Printable datasheet for Apaf1 Antibody (OAPB00039) (OAPB00039) |
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Predicted Species Reactivity | Human|Mouse|Rat |
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Product Format | Liquid. Apaf1 Antibody is supplied in PBS containing 0.02% sodium azide. |
Clonality | Polyclonal |
Host | Rabbit |
Conjugation | Unconjugated |
Application | Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot |
:: | Buffer: Antibody is supplied in PBS containing 0.02% sodium azide. |
Reconstitution and Storage | Apaf1 antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Immunogen | Apaf1 antibody was raised against a peptide corresponding to amino acids near the amino terminus of human Apaf1. The sequences of the immunogenic peptide are identical between human and mouse. The immunogen is located within the first 50 amino acids of Apaf1. |
Purification | Affinity Purified |
Predicted Homology Based on Immunogen Sequence | Human; Mouse; Rat |
Concentration | 1 mg/ml |
Blocking Peptide | For anti-Apaf1 antibody is Catalog # OEPB02167 |
Application Info | Apaf1 antibody can be used for detection of Apaf1 by Western blot at 1 - 2 ug/ml. (Optimal dilution should be determined by user. ) Whole cell lysate from HeLa cells can be used as positive control and a 115 - 130 kDa band should be detected. (Optimal dilution should be determined by user) |
Reference | Zou H, Henzel WJ, Liu X, Lutschg A, Wang X. Apaf1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3. Cell 1997;90:405-13 ;Cecconi F, Alvarez-Bolado G, Meyer BI, Roth KA, Gruss P. Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development. Cell 1998;94:727-37 ;Li P, Nijhawan D, Budihardjo I, Srinivasula SM, Ahmad M, Alnemri ES, Wang X. Cytochrome c and dATP-dependent formation ofApaf1/caspase-9 complex initiates an apoptotic protease cascade. Cell 1997;91:479-89 ;Hu Y, Benedict MA, Wu D, Inohara N, Nunez G. Bcl-XL interacts with Apaf1 and inhibits Apaf1-dependent caspase-9 activation. Proc Natl Acad Sci USA 1998;95:4386-91 (RD1299) |
Gene Symbol | APAF1 |
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Gene Full Name | apoptotic peptidase activating factor 1 |
Alias Symbols | APAF-1;apoptotic protease-activating factor 1;CED4. |
NCBI Gene Id | 317 |
Protein Name | apoptotic protease activating factor 1 [Homo sapiens]|Apoptotic protease-activating factor 1 |
Description of Target | Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis. |
Uniprot ID | O14727 |
Protein Accession # | https://www.ncbi.nlm.nih.gov/protein/AAC51678 |
Molecular Weight | Predicted: 132 kDa Observed: 130 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Apaf1 Antibody (OAPB00039)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "Apaf1 Antibody (OAPB00039)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "Apaf1 Antibody (OAPB00039)" provided in?
This item is provided in "Liquid. Apaf1 Antibody is supplied in PBS containing 0.02% sodium azide.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Apaf1 Antibody (OAPB00039)"?
This target may also be called "APAF-1;apoptotic protease-activating factor 1;CED4." in publications.
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What is the shipping cost for "Apaf1 Antibody (OAPB00039)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Apaf1 Antibody (OAPB00039)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Apaf1 Antibody (OAPB00039)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "Predicted: 132 kDa Observed: 130 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Apaf1 Antibody (OAPB00039)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "APAF1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "APAF1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "APAF1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "APAF1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "APAF1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "APAF1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.