AP2M1 Antibody (OAGA00098)

Catalog No: OAGA00098

Size:100 ul

Sample(30 ?g of whole cell lysate)
A:293T
B:HeLa S3
10% SDS PAGE
OAGA00098 diluted at 1:500

Immunohistochemical analysis of paraffin-embedded N87 xenograft, using AP2M1(OAGA00098) antibody at 1:100 dilution.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IHC-FFPE, WB
• Additional Information: Background: This gene encodes a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. The encoded protein is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. The encoded protein may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
  Positive Controls: 293T, A431, H1299, HeLaS3, Raji
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 150 (Q96CW1) of AP2M1
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.57 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: AP2M1
• Gene Full Name: AP-2 complex subunit mu
• Alias Symbols: mu2, AP50, MRD60, CLAPM1
• NCBI Gene Id: 1173
• Protein Name: AP-2 complex subunit mu
• Uniprot ID: Q96CW1
• Protein Accession #: NP_001020376.1
• Molecular Weight: 50 kDa