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AP2M1 Antibody - Middle region : FITC (ARP73747_P050-FITC)
Datasheets/Manuals | Printable datasheet for anti-AP2M1 (ARP73747_P050-FITC) antibody |
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Predicted Species Reactivity | Rat |
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Product Format | Liquid. Purified antibody supplied in 1x PBS buffer. |
Clonality | Polyclonal |
Host | Rabbit |
Conjugation | FITC: Fluorescein Isothiocyanate |
Application | WB |
Reconstitution and Storage | All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding. |
Immunogen | The immunogen is a synthetic peptide directed towards the Middle region of Rat AP2M1 |
Purification | Affinity purified |
Peptide Sequence | Synthetic peptide located within the following region: YLSGMPECKFGMNDKIVIEKQGKGTADETSKSGKQSIAIDDCTFHQCVRL |
Concentration | 0.5 mg/ml |
Blocking Peptide | For anti-AP2M1 (ARP73747_P050-FITC) antibody is Catalog # AAP73747 |
Gene Symbol | AP2M1 |
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Alias Symbols | mu2, Ap50, Clapm1 |
NCBI Gene Id | 116563 |
Description of Target | AP2M1 is a component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. It is an adaptor protein which complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin- coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also . |
Uniprot ID | P84092 |
Protein Accession # | NP_446289.1 |
Protein Size (# AA) | 435 |
Molecular Weight | 47 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "AP2M1 Antibody - Middle region : FITC (ARP73747_P050-FITC)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Rat".
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How long will it take to receive "AP2M1 Antibody - Middle region : FITC (ARP73747_P050-FITC)"?
This item is available "Domestic: within 24 hours delivery | International: 3-5 days".
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What buffer format is "AP2M1 Antibody - Middle region : FITC (ARP73747_P050-FITC)" provided in?
This item is provided in "Liquid. Purified antibody supplied in 1x PBS buffer.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "AP2M1 Antibody - Middle region : FITC (ARP73747_P050-FITC)"?
This target may also be called "mu2, Ap50, Clapm1" in publications.
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What is the shipping cost for "AP2M1 Antibody - Middle region : FITC (ARP73747_P050-FITC)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "AP2M1 Antibody - Middle region : FITC (ARP73747_P050-FITC)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "AP2M1 Antibody - Middle region : FITC (ARP73747_P050-FITC)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "47 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "AP2M1 Antibody - Middle region : FITC (ARP73747_P050-FITC)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "AP2M1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "AP2M1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "AP2M1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "AP2M1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "AP2M1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "AP2M1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.