AP2M1 Antibody - C-terminal region (OAAB10967)

Catalog No: OAAB10967

Size:400ul

AP2M1 Antibody (C-term) (Cat. #OAAB10967) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the AP2M1 antibody detected the AP2M1 protein (arrow).

AP2M1 Antibody (C-term) (Cat. #OAAB10967) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Tested Species Reactivity: Human, Mouse
• Predicted Species Reactivity: Rat, Bovine, Chicken, Monkey, Xenopus, Zebrafish, C.Elegans
• Product Format: LiquidPBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, FC, ELISA
• Additional Information: Cellular Location: Cell membrane. Membrane, coated pit; Peripheral membrane protein; Cytoplasmic side. Note=AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This AP2M1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 387-415 amino acids from the C-terminal region of human AP2M1.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: 0.5 mg/ml
• Application Info: WB: 1:1000
  FC: 1:10~50

Target Info

• Gene Symbol: AP2M1
• Alias Symbols: mu2, AP50, MRD60, CLAPM1
• NCBI Gene Id: 1173
• Protein Name: AP-2 complex subunit mu
• Description of Target: This gene encodes a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. The encoded protein is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. The encoded protein may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein. Two transcript variants encoding different isoforms have been found for this gene.
• Uniprot ID: Q96CW1
• Protein Accession #: NP_001020376.1
• Molecular Weight: 50 kDa