AP2B1 ELISA Kit (Human) (OKEH02228)

Catalog No: OKEH02228

Size:96T

Human AP2B1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology AP2B1 ELISA Kit (Human) (OKEH02228) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for AP2B1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for AP2B1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample AP2B1 captured in well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 5.6% (n = 20)
  Mean Inter-assay CV%: < 9.2% (n = 20)
• ELISA Kit Component:
  • AP2B1 Microplate: 96 Wells (12 x 8 Well strips)
  • AP2B1 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated AP2B1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.11 ng/mL
• Specificity: Natural and recombinant Human AP-2 complex subunit beta
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: AP2B1
• Gene Full Name: adaptor related protein complex 2 subunit beta 1
• Alias Symbols: adapter-related protein complex 2 beta subunit;adapter-related protein complex 2 subunit beta;adaptin, beta 2 (beta);adaptor protein complex AP-2 subunit beta;adaptor related protein complex 2 beta 1 subunit;adaptor-related protein complex 2 subunit beta;ADTB2;AP105B;AP-2 complex subunit beta;AP2-BETA;beta2-adaptin;beta-2-adaptin;beta-adaptin;CLAPB1;clathrin assembly protein complex 2 beta large chain;clathrin-associated/assembly/adaptor protein, large, beta 1;plasma membrane adaptor HA2/AP2 adaptin beta subunit;testicular tissue protein Li 22.
• NCBI Gene Id: 163
• Protein Name: P63010
• Description of Target: AP-2 complex subunit beta
• Uniprot ID: https://www.uniprot.org/uniprot/P63010
• Protein Accession #: NP_001025177.1
• Nucleotide Accession #: NM_001030006.1
• Protein Size (# AA): 937
• Molecular Weight: 105 kDa