AOX1 Antibody (OAAF03253)

Catalog No: OAAF03253 (Formerly GWB-ASE336)

Size:100 ug

Western blot analysis of extracts from HeLa cells, using AOX1 Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using AOX1 Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human AOX1.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: AOX1 antibody detects endogenous levels of total AOX1 protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1:3000; IHC: 1:50~1:100; ELISA: 1:40000
• Reference: Wright R.M., Proc. Natl. Acad. Sci. U.S.A. 90:10690-10694(1993).
  Wright R.M., Redox Rep. 3:135-144(1997).
  Hillier L.W., Nature 434:724-731(2005).

Target Info

• Gene Symbol: AOX1
• Gene Full Name: aldehyde oxidase 1
• Alias Symbols: aldehyde oxidase;Aldehyde oxidase 1;AO;AOH1;azaheterocycle hydroxylase.
• NCBI Gene Id: 316
• Protein Name: Aldehyde oxidase
• Description of Target: Oxidase with broad substrate specificity, oxidizing aromatic azaheterocycles, such as N1-methylnicotinamide, N-methylphthalazinium and phthalazine, as well as aldehydes, such as benzaldehyde, retinal, pyridoxal, and vanillin. Plays a key role in the metabolism of xenobiotics and drugs containing aromatic azaheterocyclic substituents. Participates in the bioactivation of prodrugs such as famciclovir, catalyzing the oxidation step from 6-deoxypenciclovir to penciclovir, which is a potent antiviral agent. Is probably involved in the regulation of reactive oxygen species homeostasis. May be a prominent source of superoxide generation via the one-electron reduction of molecular oxygen. Also may catalyze nitric oxide (NO) production via the reduction of nitrite to NO with NADH or aldehyde as electron donor. May play a role in adipogenesis.
• Uniprot ID: Q06278
• Protein Accession #: NP_001150.3
• Molecular Weight: 147 kDa