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Catalog No: OKEH03283
Size:96T
Price: $525.00
SKU
OKEH03283
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology AOC3 ELISA Kit (Mouse) (OKEH03283) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Aoc3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Aoc3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Aoc3 captured in well.
ELISA Kit Range31.2-2000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 103%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 6.3% (n = 20)
Mean Inter-assay CV%: <= 8.2% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Aoc3 Microplate96 Wells (12 x 8 Well strips)
Aoc3 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Aoc3 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity15.6 pg/mL
SpecificityNatural and recombinant Mouse Membrane primary amine oxidase
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolAoc3
Gene Full Nameamine oxidase, copper containing 3
Alias Symbolscopper amine oxidase;membrane primary amine oxidase;semicarbazide-sensitive amine oxidase;SSAO;V;VAP1;VAP-1;vascular adhesion protein 1.
NCBI Gene Id11754
Protein NameO70423
Description of TargetMembrane primary amine oxidase
Uniprot IDhttps://www.uniprot.org/uniprot/O70423
Protein Accession #NP_033805.1
Nucleotide Accession #NM_009675.2
Protein Size (# AA)765
Molecular Weight85 kDa
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