AOC3 ELISA Kit (Mouse) (OKCD08074)

Catalog No: OKCD08074

Size:96WELLS

AOC3 ELISA Kit (Mouse) (OKCD08074) Standard Curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Amine Oxidase, Copper Containing 3 (AOC3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Amine Oxidase, Copper Containing 3 (AOC3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Amine Oxidase, Copper Containing 3 (AOC3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Amine Oxidase, Copper Containing 3 (AOC3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Amine Oxidase, Copper Containing 3 (AOC3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Amine Oxidase, Copper Containing 3 (AOC3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • AOC3 Microplate: 96 Wells (12 x 8 Well strips)
  • AOC3 Lyophilized Standard: 2
  • 100X AOC3 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 0.65ng/mL

Target Info

• Gene Symbol: Aoc3
• Gene Full Name: amine oxidase, copper containing 3
• Alias Symbols: copper amine oxidase;membrane primary amine oxidase;semicarbazide-sensitive amine oxidase;SSAO;V;VAP1;VAP-1;vascular adhesion protein 1.
• NCBI Gene Id: 11754
• Protein Name: Membrane primary amine oxidase
• Description of Target: Cell adhesion protein that participates in lymphocyte recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin-independent fashion. Has a monoamine oxidase activity. May play a role in adipogenesis (By similarity).
• Uniprot ID: O70423
• Protein Accession #: NP_033805.1
• Nucleotide Accession #: NM_009675.2