AOC3 Antibody - middle region (OAAB05130)

Catalog No: OAAB05130

Size:400ul

Western blot analysis of AOC3 Antibody (Center) (Cat. #OAAB05130) in CEM cell line lysates (35ug/lane).AOC3 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human colon carcinoma with AOC3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometric analysis of CEM cells using AOC3 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB, IHC, FC
• Additional Information: Function: Cell adhesion protein that participates in lymphocyte extravasation and recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin-independent fashion. Has semicarbazide-sensitive (SSAO) monoamine oxidase activity. May play a role in adipogenesis.
  Cellular Location: Cell membrane; Single-pass type II membrane protein
  Tissue Location: Strongly expressed on the high endothelial venules of peripheral lymph nodes and on hepatic endothelia. Also highly expressed in appendix, lung and small intestine. Expressed also in adipose tissue, in bone marrow, colon, heart, kidney, ovary, pancreas, placenta, prostate, skeletal muscle, spleen and testis. Isoform 2 seems to be the predominant transcript in fetal kidneys, fetal cartilage and fetal tonsils. The highest relative expression of isoform 2 occurs in skeletal muscle, heart, pancreas, kidney, and lung
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This AOC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 613-640 amino acids from the Central region of human AOC3.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: IHC-P: 1:25
  WB: 1:2000
  WB: 1:1000
  IHC-P: 1:10~50
  FC: 1:10~50
• Other Applications Image 1 Data: Flow cytometric analysis of CEM cells using AOC3 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Target Info

• Gene Symbol: AOC3
• Alias Symbols: HPAO, SSAO, VAP1, VAP-1
• NCBI Gene Id: 8639
• Protein Name: Membrane primary amine oxidase
• Uniprot ID: Q16853
• Protein Accession #: NP_003725.1
• Molecular Weight: 84622 Da