ANXA6 ELISA Kit (Human) (OKCD08095)

Catalog No: OKCD08095

Size:96WELLS

ANXA6 ELISA Kit (Human) (OKCD08095) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Annexin A6 (ANXA6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Annexin A6 (ANXA6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Annexin A6 (ANXA6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Annexin A6 (ANXA6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Annexin A6 (ANXA6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Annexin A6 (ANXA6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • ANXA6 Microplate: 96 Wells (12 x 8 Well strips)
  • ANXA6 Lyophilized Standard: 2
  • 100X ANXA6 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.056ng/mL

Target Info

• Gene Symbol: ANXA6
• Gene Full Name: annexin A6
• Alias Symbols: 67 kDa calelectrin;annexin A6;Annexin VI;annexin VI (p68);annexin-6;ANX6;calcium-binding protein p68;calelectrin;calphobindin II;Calphobindin-II;CBP68;chromobindin-20;CPB-II;lipocortin VI;p68;p70;Protein III;testis secretory sperm-binding protein Li 198a.
• NCBI Gene Id: 309
• Protein Name: Annexin A6
• Description of Target: May associate with CD21. May regulate the release of Ca(2+) from intracellular stores.
• Uniprot ID: P08133
• Protein Accession #: NP_001146.2
• Nucleotide Accession #: NM_001155.4