ANXA5 ELISA Kit (Human) (OKCD06186)

Catalog No: OKCD06186

Size:96WELLS

ANXA5 ELISA Kit (Human) (OKCD06186) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Annexin V (ANXA5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Annexin V (ANXA5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Annexin V (ANXA5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Annexin V (ANXA5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 31.2-2,000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Annexin V (ANXA5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Annexin V (ANXA5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • ANXA5 Microplate: 96 Wells (12 x 8 Well strips)
  • ANXA5 Lyophilized Standard: 2
  • 100X ANXA5 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 14.1pg/mL

Target Info

• Gene Symbol: ANXA5
• Gene Full Name: annexin A5
• Alias Symbols: anchorin CII;annexin A5;annexin V;annexin-5;ANX5;calphobindin I;CBP-I;endonexin II;ENX2;epididymis secretory protein Li 7;HEL-S-7;lipocortin V;PAP-I;placental anticoagulant protein 4;placental anticoagulant protein I;PP4;RPRGL3;thromboplastin inhibitor;VAC-alpha;vascular anticoagulant-alpha.
• NCBI Gene Id: 308
• Protein Name: Annexin A5
• Description of Target: This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
• Uniprot ID: P08758
• Protein Accession #: NP_001145.1
• Nucleotide Accession #: NM_001154.3