ANXA5 Antibody (OAGA02112)

Catalog No: OAGA02112

Size:100 ul

Annexin V antibody detects ANXA5 protein by Western blot analysis.
A. 30 ug PC-12 whole cell lysate/extract
B. 30 ug Rat2 whole cell lysate/extract
10 % SDS-PAGE
Annexin V antibody (OAGA02112) dilution: 1:1000

Sample (30 ug of whole cell lysate)
A:NIH-3T3
10% SDS PAGE
OAGA02112 diluted at 1:1000

Sample (30 ug of whole cell lysate)
A:HeLa S3
B:Hep G2
12% SDS PAGE
OAGA02112 diluted at 1:2000

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Annexin V (OAGA02112) antibody at 1:500 dilution.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IF, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Annexin V. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.39mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Target Info

• Gene Symbol: ANXA5
• Gene Full Name: annexin A5
• Alias Symbols: PP4, ANX5, ENX2, RPRGL3, HEL-S-7
• NCBI Gene Id: 308
• Description of Target: The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa.
• Uniprot ID: P08758