ANXA4 ELISA Kit (Pig) (OKEH08418)

Catalog No: OKEH08418

Size:96T

Pig ANXA4 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Porcine, Sus scrofa
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  serum(n=5)	83-83% (n = 20)	111-120% (n = 20)	105-115% (n = 20)	104-114% (n = 20)
  EDTA plasma(n=5)	84-93% (n = 20)	105-114% (n = 20)	83-93% (n = 20)	97-107% (n = 20)
  heparin plasma(n=5)	85-94% (n = 20)	108-118% (n = 20)	113-122% (n = 20)	109-118% (n = 20)

• ELISA Kit Principle: Aviva Systems Biology ANXA4 ELISA Kit (Pig) (OKEH08418) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ANXA4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ANXA4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ANXA4 captured in well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=4.7% (n = 20)
  Mean Inter-assay CV%: <=8.8% (n = 20)
• ELISA Kit Component:
  • ANXA4 Microplate: 96 Wells (12 x 8 Well strips)
  • ANXA4 Lyophilized Standard: 2
  • 100X Biotinylated ANXA4 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis.
  Unigene: Ssc.1143
  SMR: P08132
  String: 9823.ENSSSCP00000008898
  KEGG: ssc:100312960
  Pfam: PF00191
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.087ng/mL
• Specificity: Natural and recombinant Pig Annexin A4
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ANXA4
• Gene Full Name: annexin A4
• Alias Symbols: 35-beta calcimedin;annexin A4;Annexin IV;Annexin-4;ANX4;Chromobindin-4;Endonexin I;Lipocortin IV;P32.5;PAP-II;Placental anticoagulant protein II;PP4-X;Protein II.
• NCBI Gene Id: 100312960
• Protein Name: P08132
• Description of Target: Annexin A4
• Uniprot ID: https://www.uniprot.org/uniprot/P08132
• Protein Accession #: NP_001161111.1
• Nucleotide Accession #: NM_001167639.1