ANXA4 ELISA Kit (Human) (OKEH04695)

Catalog No: OKEH04695

Size:96T

Human ANXA4 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology ANXA4 ELISA Kit (Human) (OKEH04695) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ANXA4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ANXA4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ANXA4 captured in well.
• ELISA Kit Range: 78-5000pg/mL
• ELISA Kit Reproducibility: Mean Intra-Assay CV: < 10%
  Mean Inter-Assay CV: < 12%
• ELISA Kit Component:
  • ANXA4 Microplate: 96 Wells (12 x 8 Well Strips)
  • ANXA4 Standard: 2
  • Sample Diluent: 1 x 20 mL
  • ANXA4 Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
• Sensitivity: 41 pg/mL
• Specificity: Natural and recombinant Human Annexin A4
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ANXA4
• Gene Full Name: annexin A4
• Alias Symbols: 35-beta calcimedin;annexin A4;Annexin IV;annexin IV (placental anticoagulant protein II);annexin-4;ANX4;carbohydrate-binding protein p33/p41;chromobindin-4;endonexin I;epididymis secretory protein Li 274;HEL-S-274;lipocortin IV;P32.5;PAP-II;PIG28;placental anticoagulant protein II;PP4-X;proliferation-inducing gene 28;proliferation-inducing protein 28;protein II;ZAP36.
• NCBI Gene Id: 307
• Protein Name: P09525
• Description of Target: Annexin A4
• Uniprot ID: https://www.uniprot.org/uniprot/P09525
• Protein Accession #: NP_001144.1
• Nucleotide Accession #: NM_001153.4
• Protein Size (# AA): 319
• Molecular Weight: 36 kDa