Catalog No: OCOA12769
Size:20UG
Price: $179.00
SKU
OCOA12769
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ANXA3 Protein Lysate (OCOA12769)

Datasheets/ManualsPrintable datasheet for ANXA3 Protein Lysate (OCOA12769)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionANXA3 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolANXA3
Gene Full Nameannexin A3
Alias Symbols35-alpha calcimedin;annexin A3;Annexin III;Annexin III (lipocortin III);annexin III (lipocortin III, 1,2-cyclic-inositol-phosphate phosphodiesterase, placental anticoagulant protein III, calcimedin 35-alpha);annexin-3;ANX3;calcimedin 35-alpha;inositol 1,2-cyclic phosphate 2-phosphohydrolase;lipocortin III;PAP-III;placental anticoagulant protein III.
NCBI Gene Id306
Protein NameNM_005139, NP_005130, P12429
Description of TargetAnnexin A3, annexin A3 [Homo sapiens], Homo sapiens annexin A3 (ANXA3), mRNA
Uniprot IDhttps://www.uniprot.org/uniprot/P12429
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_005130
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_005139
Molecular Weight36.4 kDa
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