ANXA2 Chemi-Luminescent ELISA Kit (Human) (OKCD03298)

Catalog No: OKCD03298

Size:96WELLS

Human ANXA2 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	82-103%	87-95%	91-99%	78-101%
  EDTA Plasma (n=5)	79-99%	81-101%	86-104%	80-99%
  Heparin Plasma (n=5)	90-98%	97-104%	88-101%	98-105%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Annexin A2 (ANXA2). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Annexin A2 (ANXA2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Annexin A2 (ANXA2) level in the sample or standard.
• ELISA Kit Range: 54.9-40,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	79-97	89
  EDTA Plasma (n=5)	82-104	95
  Heparin Plasma (n=5)	93-101	96

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Annexin A2 (ANXA2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Annexin A2 (ANXA2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • ANXA2 Microplate: 96 Wells (12 x 8 Well strips)
  • ANXA2 Lyophilized Standard: 2
  • 100X ANXA2 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.625 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ANXA2
• Gene Full Name: annexin A2
• Alias Symbols: annexin A2;annexin II;annexin-2;ANX2;ANX2L4;CAL1H;calpactin I heavy chain;calpactin I heavy polypeptide;calpactin-1 heavy chain;chromobindin 8;Chromobindin-8;epididymis secretory protein Li 270;epididymis secretory sperm binding protein;HEL-S-270;LIP2;lipocortin II;LPC2;LPC2D;P36;PAP-IV;placental anticoagulant protein IV;protein I.
• NCBI Gene Id: 302
• Protein Name: Annexin A2
• Description of Target: Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. Inhibits PCSK9-enhanced LDLR degradation, probably reduces PCSK9 protein levels via a translational mechanism but also competes with LDLR for binding with PCSK9 (PubMed:18799458, PubMed:24808179, PubMed:22848640).
• Uniprot ID: P07355
• Protein Accession #: NP_001002857
• Nucleotide Accession #: NM_001002857