ANXA1 ELISA Kit (Human) (OKBB01211)

Catalog No: OKBB01211

Size:96 wells

ANXA1 ELISA Kit (Human) (OKBB01211) Standard Curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva's human Annexin A1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Annexin A1 has been precoated onto 96-well plates. Standards(Expression system for standard: NS0, Immunogen sequence: A2-N346) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Annexin A1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Fibronectin amount of sample captured in plate.
• ELISA Kit Range: 156 pg/ml - 10,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean(pg/ml)	299	1474	5209	303	1499	5311
  Standard deviation	12.56	64.86	276.1	17.9	92.9	414.3
  CV(%)	4.2	4.4	5.3	5.9	6.2	7.8

• ELISA Kit Component:
  • 96-Well Plate Pre-coated with Anti-Human ANXA1 Antibody: 96 Wells (12 x 8 Well Strips)
  • Lyophilized Recombinant Human ANXA1 Standard: 2 x 10 ng
  • 100X Biotinylated Anti-Human ANXA1 Antibody: 1 x 130 uL
  • 100X Avidin-Biotin Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Additional Information:

  To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

  Lots	Lot1 (pg/ml)	Lot2 (pg/ml)	Lot3 (pg/ml)	Lot4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	299	282	293	299	293	6.94	2.3%
  Sample 2	1474	1425	1389	1444	1433	30.83	2.1%
  Sample 3	5209	4826	5016	4992	5010	135.84	2.7%

  *number of samples for each test n=16.

  
  

  Standard Number	8	7	6	5	4	3	2	1
  Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
  O.D.	0.066	0.196	0.245	0.452	0.861	1.437	1.943	2.275

• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: cell culture supernates, serum and plasma (heparin, EDTA)
• Sensitivity: <100 pg/ml
• Specificity: Natural and recombinant human Annexin A1
• Immunogen: Expression system for standard: NS0; Immunogen sequence: A2-N346
• Description: For quantitative detection of human Annexin A1 in cell culture supernatants, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: ANXA1
• Gene Full Name: annexin A1
• Alias Symbols: Annexin A1; Annexin I; Annexin-1; Calpactin II; Calpactin-2; Chromobindin-9; Lipocortin I; Phospholipase A2 inhibitory protein; p35; ANXA1; ANX1; LPC1
• NCBI Gene Id: 301
• Protein Name: Annexin A1
• Description of Target: ANXA1, also known as lipocortin I or Annexin A1, is a protein that in humans is encoded by the ANXA1 gene. It is mapped to 9q21.13. ANXA1 belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. ANXA1 protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Lower peptide concentrations possibly found in inflammatory situations elicit Ca(2+) transients without fully activating the mitogen-activated protein kinase pathway. This causes a specific inhibition of the transendothelial migration of neutrophils and a desensitization of neutrophils toward a chemoattractant challenge. These findings identified ANXA1 peptides as novel, endogenous FPR ligands and established a mechanistic basis of ANXA1-mediated antiinflammatory effects.
• Uniprot ID: P04083
• Protein Accession #: NP_000691.1
• Nucleotide Accession #: NM_000700.2