ANXA1 ELISA Kit (Guinea pig) (OKCA01051)

Catalog No: OKCA01051

Size:96 Wells

Guinea Pig ANXA1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Guinea Pig
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean Recovery (%)	Range (%)
  Neat	89	82-95
  1:2	95	86-105
  1:4	93	86-99
  1:8	97	86-100

• ELISA Kit Principle: Aviva Systems Biology ANXA1 ELISA Kit (Guinea pig) (OKCA01051) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ANXA1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ANXA1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample ANXA1 captured in the well.
• ELISA Kit Range: 7.8 - 500 ng/mL
• ELISA Kit Recovery:

  Sample	Mean Recovery (%)	Range (%)
  Serum (n=5)	92	89-100
  EDTA Plasma (n=4)	93	85-96

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level OKCA01051 were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level OKCA01051 were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • ANXA1 Microplate: 96 Wells (12 x 8 Well strips)
  • ANXA1 Lyophilized Standard: 2 x 500 ng
  • 100X Biotinylated ANXA1 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 1.95 ng/mL
• Assay Info: Assay Methodology: Quantitative Sanadwich ELISA

Target Info

• Gene Symbol: ANXA1
• Gene Full Name: Annexin A1
• Alias Symbols: ANX1, LPC1, OTTHUMP00000021475|annexin I|annexin I (lipocortin I)|lipocortin I
• Protein Name: Annexin A1
• Description of Target: Plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. Has anti-inflammatory activity. Plays a role in glucocorticoid-mediated down-regulation of the early phase of the inflammatory response. Promotes resolution of inflammation and wound healing. Functions at least in part by activating the formyl peptide receptors and downstream signaling cascades. Promotes chemotaxis of granulocytes and monocytes via activation of the formyl peptide receptors. Contributes to the adaptive immune response by enhancing signaling cascades that are triggered by T-cell activation, regulates differentiation and proliferation of activated T-cells. Promotes the differentiation of T-cells into Th1 cells and negatively regulates differentiation into Th2 cells. Has no effect on unstimulated T-cells. Promotes rearrangement of the actin cytoskeleton, cell polarization and cell migration. Negatively regulates hormone exocytosis via activation of the formyl peptide receptors and reorganization of the actin cytoskeleton. Has high affinity for Ca2+ and can bind up to eight Ca2+ ions. Displays Ca2+-dependent binding to phospholipid membranes (PubMed:2962884). Plays a role in the formation of phagocytic cups and phagosomes. Plays a role in phagocytosis by mediating the Ca2+-dependent interaction between phagosomes and the actin cytoskeleton.
• Uniprot ID: P14087