ANXA1 Chemi-Luminescent ELISA Kit (Human) (OKCD03297)

Catalog No: OKCD03297

Size:96WELLS

Human ANXA1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	96-103%	97-105%	90-99%	87-96%
  EDTA Plasma (n=5)	93-101%	78-98%	82-98%	93-102%
  Heparin Plasma (n=5)	91-98%	78-93%	79-102%	93-101%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Annexin A1 (ANXA1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Annexin A1 (ANXA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Annexin A1 (ANXA1) level in the sample or standard.
• ELISA Kit Range: 0.27-200ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	85-103	89
  EDTA Plasma (n=5)	93-104	101
  Heparin Plasma (n=5)	91-101	96

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Annexin A1 (ANXA1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Annexin A1 (ANXA1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • ANXA1 Microplate: 96 Wells (12 x 8 Well strips)
  • ANXA1 Lyophilized Standard: 2
  • 100X ANXA1 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 3.12 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ANXA1
• Gene Full Name: annexin A1
• Alias Symbols: annexin A1;Annexin I;annexin I (lipocortin I);annexin-1;ANX1;calpactin II;calpactin-2;chromobindin-9;epididymis secretory sperm binding protein;Lipocortin I;LPC1;p35;phospholipase A2 inhibitory protein.
• NCBI Gene Id: 301
• Protein Name: Annexin A1
• Description of Target: Plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. Has anti-inflammatory activity (PubMed:8425544). Plays a role in glucocorticoid-mediated down-regulation of the early phase of the inflammatory response (By similarity). Promotes resolution of inflammation and wound healing (PubMed:25664854). Functions at least in part by activating the formyl peptide receptors and downstream signaling cascades (PubMed:15187149, PubMed:25664854). Promotes chemotaxis of granulocytes and monocytes via activation of the formyl peptide receptors (PubMed:15187149). Contributes to the adaptive immune response by enhancing signaling cascades that are triggered by T-cell activation, regulates differentiation and proliferation of activated T-cells (PubMed:17008549). Promotes the differentiation of T-cells into Th1 cells and negatively regulates differentiation into Th2 cells (PubMed:17008549). Has no effect on unstimulated T cells (PubMed:17008549). Promotes rearrangement of the actin cytoskeleton, cell polarization and cell migration (PubMed:15187149). Negatively regulates hormone exocytosis via activation of the formyl peptide receptors and reorganization of the actin cytoskeleton (PubMed:19625660). Has high affinity for Ca(2+) and can bind up to eight Ca(2+) ions (By similarity). Displays Ca(2+)-dependent binding to phospholipid membranes (PubMed:2532504, PubMed:8557678). Plays a role in the formation of phagocytic cups and phagosomes. Plays a role in phagocytosis by mediating the Ca(2+)-dependent interaction between phagosomes and the actin cytoskeleton (By similarity).
• Uniprot ID: P04083
• Protein Accession #: NP_000691
• Nucleotide Accession #: NM_000700