Anti-Voltage Gated Potassium Channel, Kv2.2 Subunit (OAPC00001)

Catalog No: OAPC00001 (Formerly GWB-88E162)

Size:100 ul

Western blot of rat brain homogenate showing specific immunolabeling of the ~125k voltage-gated potassium channel, Kv2.2 (Control). The immunolabeling is blocked by preadsorption with the peptide used as antigen (Peptide block).

Product Info

• Predicted Species Reactivity: Rat
• Product Format: Liquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB, IHC
• Additional Information: Packaging :
  100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Reconstitution and Storage: For long term storage -20C is recommended. Stable at -20C for at least 1 year.
• Immunogen: Peptide from the subunit region of the voltage gated potassium channel, Kv2.2 of rat and Xenopus, conjugated to keyhole limpet hemocyanin (KLH).
• Purification: Prepared from rabbit serum by affinity purification using a Sulfo-Link column matrix to which the peptide immunogen was coupled.
• Predicted Homology Based on Immunogen Sequence: Rat
• Specificity: Specific for the ~125k voltage gated potassium channel, Kv 2.2 subunit.
• Application Info: The antibody has been directly tested for reactivity in Western blots in rat tissues.
  Western blot of rat brain homogenate showing specific immunolabeling of the ~125k voltage-gated potassium channel, Kv2.2 (Control). The immunolabeling is blocked by preadsorption with the peptide used as antigen (Peptide block).
  
• Reference: Blaine JT, Ribera AB (1998) Heteromultimeric potassium channels formed by members of the Kv2 subfamily. J Neurosci 18:9585-9593.
  Burger C, Ribera AB (1996) Xenopus spinal neurons express Kv2 potassium channel transcripts during embryonic development. J Neurosci 16:1412-1421.
  Hwang PM, Fotuhi M, Bredt DS, Cunningham AM, Snyder SH (1993) Contrasting immunohistochemical localizations in rat brain of two novel K+ channels of the Shab subfamily. J Neurosci 13:1569-1576.
  Michaelevski I, Chikvashvili D, Tsuk S, Singer-Lahat D, Kang YH, Linial M, Gaisano HY, Fili O, Lotan I (2003) Direct interaction of target SNAREs with the Kv2.1 channel - Modal regulation of channel activation and inactivation gating. J Biol Chem 278:34320-34330.
  Pongs O (1999) Voltage-gated potassium channels: from hyperexcitability to excitement. FEBS Lett 452:31-35.
  Yan L, Figueroa DJ, Austin CP, Liu Y, Bugianesi RM, Slaughter RS, Kaczorowski GJ, Kohler MG (2004) Expression of voltage-gated potassium channels in human and rhesus pancreatic islets. Diabetes 53:597-607.
  D. Guan, T. Tkatch, D. J. Surmeier, W. E. Armstrong, and R. C. Foehring (2007) Kv2 subunits underlie slowly inactivating potassium current in rat neocortical pyramidal neurons J. Physiol., Jun 2007; 581: 941 - 960.

Target Info

• Gene Symbol: KCNB2
• NCBI Gene Id: 621349
• Protein Name: Potassium voltage-gated channel subfamily B member 2
• Description of Target: Voltage-gated K+ channels are important determinants of neuronal membrane excitability (Pongs, 1999). Moreover, differences in K+ channel expression patterns and densities contribute to the variations in action potential waveforms and repetitive firing patterns evident in different neuronal cell types. The delayed rectifier-type (IK)channels (Kv1.5, Kv2.1, and Kv2.2) are expressed on all neuronal somata and proximal dendrites and are also found in a wide variety of non-neuronal cells types including pancreatic islets, alveolar cells and cardiac myocytes (Hwang et al., 1993; Yan et al., 2004; Michaelevski et al., 2003). Kv2.1 and Kv2.2 form distinct populations of K+ channels and these subunits are thought to be primarily responsible for IK in superior cervical ganglion cells (Blaine and Ribera, 1998; Burger and Ribera, 1996).
• Uniprot ID: Q63099
• Molecular Weight: ~100kDa