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Datasheets/Manuals | Printable datasheet for OAPC00086 |
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Predicted Species Reactivity | Human, Rat |
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Product Format | Liquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots. |
Clonality | Polyclonal |
Host | Sheep |
Application | WB |
Additional Information | Packaging : 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots. |
Reconstitution and Storage | Store at -20C in undiluted aliquots; stable for at least 1 year. Avoid freeze/thaw cycles. |
Immunogen | Peptide from intracellular C-terminal region of human VMAT2, conjugated to keyhole limpet hemocyanin (KLH). |
Purification | Prepared from sheep serum by affinity purification using a column to which the peptide immunogen was coupled. |
Predicted Homology Based on Immunogen Sequence | Human; Rat |
Specificity | Specific for the ~57k VMAT2 protein in Western blots of rat caudate lysate. |
Application Info | The antibody has been directly tested for reactivity in Western blots in rat tissue. Western blot of rat caudate lysate showing specific immunolabeling of the ~ 57k VMAT2 protein. |
Reference | Erickson JD, Schafer MKH, Bonner TI, Eiden LE, Weihe E (1996) Distinct pharmacological properties and distribution in neurons and endocrine cells of two isoforms of the human vesicular monoamine transporter. Proc Natl Acad Sci USA 93:5166-5171. Giros B, El Mestikawy S, Godinot N, Zheng K, Han H, Yang-Feng T, Caron MG (1991) Cloning, pharmacological characterization, and chromosome assignment of the human dopamine transporter. J Pharmacol Exptl Ther 56:2139-2142. Haycock JW, Becker L, Ang, L, Furukawa Y, Hornykiewicz O, Kish SJ (2003) Marked disparity between age-related changes in dopamine and other presynaptic dopaminergic markers in human striatum. J Neurochem 87:574-585. Witkovsky P, Arango-Gonzalez B, Haycock JW, Kohler K (2004) Rat retinal dopaminergic neurons: Differential maturation of somatodendritic and axonal compartments. J Comp Neurol 481:352-362. |
Gene Symbol | VMAT2 |
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Alias Symbols | SVAT, SVMT, VAT2, VMAT2, PKDYS2 |
NCBI Gene Id | 6571 |
Protein Name | Synaptic vesicular amine transporter |
Description of Target | Vesicular neurotransmitter transporters sequester the transmitters into synaptic vesicles (Erickson et al., 1996). The vesicular monoamine transporter 2 (VMAT2) is responsible for catecholamine and serotonin storage in central synapses. Antibodies specific for VMAT have been used to monitor expression of the transporter during development and in aging and can be effectively used as a marker for monoamine terminals (Haycock et al., 2003; Witkovsky et al., 2005). |
Uniprot ID | Q05940 |
Protein Accession # | NP_003045 |
Nucleotide Accession # | NM_003054 |
Molecular Weight | ~57kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Anti-Vesicular Monoamine Transporter 2, C-Terminus (OAPC00086)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Rat".
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How long will it take to receive "Anti-Vesicular Monoamine Transporter 2, C-Terminus (OAPC00086)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "Anti-Vesicular Monoamine Transporter 2, C-Terminus (OAPC00086)" provided in?
This item is provided in "Liquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Anti-Vesicular Monoamine Transporter 2, C-Terminus (OAPC00086)"?
This target may also be called "SVAT, SVMT, VAT2, VMAT2, PKDYS2" in publications.
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What is the shipping cost for "Anti-Vesicular Monoamine Transporter 2, C-Terminus (OAPC00086)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Anti-Vesicular Monoamine Transporter 2, C-Terminus (OAPC00086)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Anti-Vesicular Monoamine Transporter 2, C-Terminus (OAPC00086)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "~57kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Anti-Vesicular Monoamine Transporter 2, C-Terminus (OAPC00086)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "VMAT2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "VMAT2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "VMAT2"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "VMAT2"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "VMAT2"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "VMAT2"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.