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Datasheets/Manuals | Printable datasheet for Anti-Tubulin beta Epitope Specific Rabbit Antibody (OAAI00219) |
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Predicted Species Reactivity | Human|Mouse|Rat |
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Product Format | Liquid. This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Host | Rabbit |
Application | Enzyme-linked immunosorbent assay|Flow cytometry|Immunohistochemistry|Immunoprecipitation|Western blot |
Additional Information | Background: Tubulin is the major building block of microtubules which is essential in cell structures, transportation and mitosis. This superfamily is composed of six distinct families. Alpha, beta, and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The abundance of Gamma tubulin is less than 1% of the level of either alpha or beta tubulin. It shares approximately 28-32% identity with alpha tubulin from various organisms and 32-36% identity with beta tubulins. The detection, localization and characterization of proteins involved in microtubule function is fundamental to the understanding of mitosis, meiosis and the microtubule cytoskeleton. |
:: | Positive Control: Hela cells, Lung tissue |
:: | Cellular Location: Cytoplasmic |
Reconstitution and Storage | The antibodies are stable for 24 months from date of receipt when stored at -20C to -70C. The antibodies can be stored at 2C-8C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles. |
Immunogen | A synthetic peptide corresponding to aa 416-430 of human tubulin beta. This sequence is identical among human, rat, mouse, bovine, guinea pig, chicken, gerbil, frog, yeast, fungi, sea urchin, Myxamoeba and plasmodium. |
Purification | Affinity Purified |
Concentration | 100ug/200ul |
Specificity | TUBB |
Formulation | This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer |
Application Info | WB: 0.1-1 ug/ml | ELISA: 0.01-0.1 ug/ml | IP: 2-5 ug/ml | IHC: 2-10 ug/ml | FC: Not tested |
Reference | Wang, HW, Nogales E. Nucleotide-dependent bending flexibility of tubulin regulates microtubule assembly. Nature 435 (7044): 911-915, 2005. |
Gene Symbol | TUBB |
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Gene Full Name | tubulin beta class I |
Alias Symbols | beta Ib tubulin;CDCBM6;CSCSC1;epididymis secretory sperm binding protein;M40;OK/SW-cl.56;TUBB1;TUBB5;tubulin beta chain;tubulin beta-1 chain;tubulin beta-5 chain;tubulin, beta polypeptide. |
NCBI Gene Id | 203068 |
Description of Target | Tubulin is the major building block of microtubules which is essential in cell structures, transportation and mitosis. This superfamily is composed of six distinct families. Alpha, beta, and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The abundance of Gamma tubulin is less than 1% of the level of either alpha or beta tubulin. It shares approximately 28-32% identity with alpha tubulin from various organisms and 32-36% identity with beta tubulins. The detection, localization and characterization of proteins involved in microtubule function is fundamental to the understanding of mitosis, meiosis and the microtubule cytoskeleton. |
Uniprot ID | P07437 |
Molecular Weight | 50 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Anti-Tubulin beta Epitope Specific Rabbit Antibody (OAAI00219)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "Anti-Tubulin beta Epitope Specific Rabbit Antibody (OAAI00219)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "Anti-Tubulin beta Epitope Specific Rabbit Antibody (OAAI00219)" provided in?
This item is provided in "Liquid. This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Anti-Tubulin beta Epitope Specific Rabbit Antibody (OAAI00219)"?
This target may also be called "beta Ib tubulin;CDCBM6;CSCSC1;epididymis secretory sperm binding protein;M40;OK/SW-cl.56;TUBB1;TUBB5;tubulin beta chain;tubulin beta-1 chain;tubulin beta-5 chain;tubulin, beta polypeptide." in publications.
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What is the shipping cost for "Anti-Tubulin beta Epitope Specific Rabbit Antibody (OAAI00219)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Anti-Tubulin beta Epitope Specific Rabbit Antibody (OAAI00219)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Anti-Tubulin beta Epitope Specific Rabbit Antibody (OAAI00219)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "50".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Anti-Tubulin beta Epitope Specific Rabbit Antibody (OAAI00219)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "TUBB"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "TUBB"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "TUBB"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "TUBB"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "TUBB"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "TUBB"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.