Anti-Synaptophysin 2 (OAPC00066)

Catalog No: OAPC00066

Size:100 ul

Western blot of rat hippocampal lysate showing specific labeling of the ~38k Synaptophysin2 protein.

Product Info

• Predicted Species Reactivity: Mouse, Rat
• Product Format: Liquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Additional Information: Packaging :
  100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Reconstitution and Storage: Store at -20C; stable for at least one year.
• Immunogen: Peptide corresponding to amino acid residues from the C-terminal region of rat Synaptophysin 2, conjugated to keyhole limpet hemocyanin (KLH).
• Purification: Prepared from rabbit serum by affinity purification via chromatography on an affinity column made with the C-terminal peptide used as antigen.
• Predicted Homology Based on Immunogen Sequence: Mouse; Rat
• Specificity: Specific for ~38k Synaptophysin 2 protein.
• Application Info: The antibody has been demonstrated to work in rat. It is anticipated that the antibody will work in mouse based on the fact that this species has 100% homology with the amino acid sequence used as antigen.
  Western blot of rat hippocampal lysate showing specific labeling of the ~38k Synaptophysin2 protein.
  
• Reference: Dai J, Ji C, Gu S, Wu Q, Wang L, Xu J, Zeng L, Ye X, Yin G, Xie Y, Mao Y (2003) Cloning and sequence analysis of the human cDNA encoding the synaptoporin, a highly conserved synaptic vesicle protein. Molecular Biology Reports 30:185-191.
  Singec I, Knoth R, Ditter M, Hagemeyer CE, Rosenbrock H, Frotscher M, Volk B (2002) Synaptic vesicle protein synaptoporin is differently expressed by subpopulations of mouse hippocampal neurons. J Comp Neurol 452(2):139-153.
  Grabs D, Bergmann M, Schuster T, Fox PA, Brich M, Gratz M (1994) Differential expression of synaptophysin and synaptoporin during pre- and postnatal development of the rat hippocampal network. Eur J Neurosci. 6(11): 1765-71..

Target Info

• Gene Symbol: SYNPR
• NCBI Gene Id: 66030
• Protein Name: Synaptoporin
• Description of Target: Synaptophysin 2, also known as Synaptoporin, is a component of the synaptic vesicle membrane and thought to play an important role in synaptic vesicle trafficking (Dai et al., 2003). It is highly homologous to Synaptophysin 1, yet differs in expression. Whereas Synaptophysin 1 is ubiquitiously expressed, Synaptophysin 2 is concentrated in the mossy fiber synapses of the hippocampus (Grabs et al., 1994; Singec et al., 2002).
• Uniprot ID: P22831
• Protein Accession #: NP_076464.1
• Molecular Weight: ~38kDa