Anti-Synaptojanin I (OAPC00065)

Catalog No: OAPC00065

Size:100 ul

Western blot of rat hippocampal lysate showing specific immunolabeling of the ~145k synaptojanin I protein.

Product Info

• Predicted Species Reactivity: Rat
• Product Format: Liquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Clonality: Monoclonal
• Isotype: IgG
• Host: Mouse
• Application: WB, IHC
• Additional Information: Packaging :
  100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Reconstitution and Storage: For long term storage -20C is recommended. Stable at -20C for at least 1 year
• Immunogen: Recombinant protein corresponding to amino acid residues from the C-terminal region of rat synaptojanin 1.
• Purification: Protein G purified culture supernatant.
• Predicted Homology Based on Immunogen Sequence: Rat
• Specificity: Specific for the ~145k synaptojanin I protein in Western blots of rat brain extracts. Immunolabeling blocked by preadsorption of antibody with the protein used to generate the antibody.
• Application Info: The antibody has been directly tested for reactivity in Western blots with rat tissue. Does not cross react with mouse.
  Western blot of rat hippocampal lysate showing specific immunolabeling of the ~145k synaptojanin I protein.
  
• Reference: Tissue-specific alternative splicing generates two synaptojanin isoforms with differential membrane binding properties (1996). Ramjaun AR, McPherson PS. J Biol Chem. Oct 4;271(40):24856-61.
  Synaptojanin 1-linked phosphoinositide dyshomeostasis and cognitive deficits in mouse models of Down's syndrome (2008).Voronov SV, Frere SG, Giovedi S, Pollina EA, Borel C, Zhang H, Schmidt C, Akeson EC, Wenk MR, Cimasoni L, Arancio O, Davisson MT, Antonarakis SE, Gardiner K, De Camilli P, Di Paolo G. Proc Natl Acad Sci U S A. Jul 8;105(27):9415-20.

Target Info

• Gene Symbol: SYNJ1
• NCBI Gene Id: 85238
• Protein Name: Synaptojanin-1
• Description of Target: Synaptojanin is a phosphatidylinositol phosphatase involved in clathrin-mediated endocytosis of synaptic vesicles. Synaptojanin 1 has two alternatively spliced isoforms; one that is ~ 145 kDA and is exclusively expressed in neurons, and the other ~ 170 kDa which is expressed in non-neuronal, peripheral tissues (Ramjaun AR & McPherson PS, 1996). The gene which encodes Synaptojanin 1, SYNJ1, has been mapped to chromosome 21 thus making it a candidate for involvement in Down's syndrome (DS). It has recently been demonstrated that Ts65Dn mice (the most commonly used model of DS) have altered phosphatidylinositol-4,5-bisphosphate metabolism. This defect is rescued by restoring SYNJ1 to disomy in the Ts65Dn mice (Voronov SV et al., 2008).
• Uniprot ID: Q62910
• Protein Accession #: NP_445928.2
• Molecular Weight: ~145kDa