Anti-Synaptobrevin (VAMP) (OAPC00064)

Catalog No: OAPC00064

Size:100 ul

Western blot of rat brain lysate showing specific immunolabeling of the ~ 16k synaptobrevin protein.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Pig, Rabbit
• Product Format: Liquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Clonality: Monoclonal
• Clone: SP10
• Isotype: IgM
• Host: Mouse
• Application: WB, IHC
• Additional Information: Packaging :
  100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Reconstitution and Storage: Store at -20C; stable for at least one year.
• Immunogen: Synaptic immunoprecipitate (crude) from human brain.
• Purification: Purified culture supernatant.
• Predicted Homology Based on Immunogen Sequence: Cow; Human; Mouse; Pig; Rabbit; Rat
• Specificity: The antibody is specific for the ~16kDa synaptobrevin protein in Western blots of rat brain lysate. The antibody has also been demonstrated to work in immunohistochemistry on formalin fixed, vibratome sections, but does not work on paraffin sections.
• Application Info: The antibody has been directly tested for reactivity in human, pig, hamster, rat, rabbit, mouse, and bovine.
  Western blot of rat brain lysate showing specific immunolabeling of the ~ 16k synaptobrevin protein.
  
• Reference: Deák F, Schoch S, Liu X, Südhof TC, Kavalali ET (2004) Synaptobrevin is essential for fast synaptic-vesicle endocytosis. Nat Cell Biol. 2004 Nov;6(11):1102-8.
  Sze CI, Bi H, Kleinschmidt-DeMasters BK, Filley CM, Martin LJ (2000) Selective regional loss of exocytotic presynaptic vesicle proteins in Alzheimer's disease brains. J Neurol Sci.175(2):81-90.
  Honer, W. G., Hu, L. & Davies, P. Human synaptic proteins with a heterogeneous distribution in cerebellum and visual cortex (1993). Brain Research 609, 9-20.

Target Info

• Gene Symbol: VAMP1/2
• Alias Symbols: SYB1, CMS25, SPAX1, VAMP-1
• NCBI Gene Id: 6843
• Protein Name: Vesicle-associated membrane protein 1
• Description of Target: Synaptobrevin (aka VAMP) is an integral membrane protein of synaptic vesicles that plays a major role in the formation of larger SNARE complexes, along with SNAP-25 and syntaxin. Synaptobrevin has been shown to be essential for two fast synapse-specific membrane trafficking processes: fast exocytosis for neurotransmitter release and fast endocytosis that mediates rapid recycling of synaptic vesicles (Deak et al., 2004). Decreased levels of synaptobrevin in human hippocampus and cortex have been correlated with cognitive defects in Alzheimer's disease (Sze et al., 2000).
• Uniprot ID: P23763
• Protein Accession #: NP_055046.1
• Molecular Weight: ~16kDa