Anti-STAT3 Antibody (Phospho-S 727) (OAAI00081)

Catalog No: OAAI00081

Size:100UG

Western Blot: The whole cell lysate derived from A431 with EGF stimulated (Lane 1) or without EGF stimulated (Lane 2) were immunoblotted by Rabbit anti STAT3 (pS727) (Cat#OAAI00081) antibody at 1:500.

Dot Blot: 1 ?g peptide was blot onto NC membrane A: STAT3 (pS727) (Phosphospecific) B: STAT3 (Paired S727) (Non phosphospeicfic) were blotted at a 1:2000 dilution by: 1: Rabbit anti-STAT3 (Paired S727) (Cat#OAAI00211), 2: Rabbit anti-STAT3 (Phospho specific), (Cat#OAAI00081).

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid. This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: Flow cytometry|Immunohistochemistry|Immunoprecipitation|Western blot
• Additional Information: Background: STAT proteins (Signal transducer and activator of transcription) belong to a family of cytoplasmic transcription factors that can be phosphorylated by a lignad binding to its cell surface receptor. The phosphorylation of STAT3 induces STAT dimerization, nuclear translocation and DNA binding. Stat3 is constitutively activated in a number of human tumors. Stat3-activated transcription seems to be regulated by serine phosphorylation at Ser727.
  Positive Control: EGF-stimulated A431
  Cellular Location: Nuclear
• Reconstitution and Storage: The antibodies are stable for 24 months from date of receipt when stored at -20C to -70C. The antibodies can be stored at 2C-8C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
• Immunogen: A synthetic peptide surrounding to the epitope -PMSPR-- with phosphorylation sites at Ser727 of STAT3 protein from human, mouse, rat, chicken dog and bovine origins.
• Purification: Affinity Purified
• Concentration: 100ug/200ul
• Target Post-Translational Modification: Phosphorylated
• Specificity: STAT3
• Formulation: This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
• Application Info: WB: 0.1-1 ug/ml | ELISA: | IP: 2-5 ug/ml | IHC: 2-10 ug/ml | FC: Not tested
• Reference: Ritsuko Matsuo et al. A new expression cloning stRategy for isolation of substRate-specific kinases by using phosphorylation site-specific Antibody. Journal of Immunological Methods. Volume 247, Issues 1-2, 1 January 2001, Pages 141-151

Target Info

• Gene Symbol: STAT3
• Gene Full Name: signal transducer and activator of transcription 3
• Alias Symbols: acute-phase response factor;ADMIO;ADMIO1;APRF;DNA-binding protein APRF;HIES;signal transducer and activator of transcription 3.
• NCBI Gene Id: 6774
• Description of Target: STAT proteins (Signal transducer and activator of transcription) belong to a family of cytoplasmic transcription factors that can be phosphorylated by a lignad binding to its cell surface receptor. The phosphorylation of STAT3 induces STAT dimerization, nuclear translocation and DNA binding. Stat3 is constitutively activated in a number of Human tumors. Stat3- activated transcription seems to be regulated by serine phosphorylation at Ser727.
  
• Uniprot ID: P40763
• Molecular Weight: 94