ST6Gal Antibody - Biotin Conjugated (OAAI00628)

Catalog No: OAAI00628

Size:100UG

WB: The whole cell lysate derived from HEK293 over-expressed ST6Gal protein was loaded in SDS- PAGE, transferred onto a NC membrane, then blotted by Rabbit anti ST6Gal antibody (cat#OAAI00627) at 1:500. An immune reactive band is observed around ~52kD. (The biotinylated antibody (Cat#OAAI00628) was confirmed by HRP- Strepavidin)

Product Info

• Predicted Species Reactivity: Drosophila|Human
• Product Format: Liquid. This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Flow cytometry|Immunohistochemistry|Immunoprecipitation|Western blot
• Additional Information: Background: Sialyltransferases are enzyme that transfer sialic acid to nascent oligosaccharide. Each silaytransferase is specific for a particular sugar substrate. Sialytransferases add sialic acid to the terminal portins of the sialylated glycolipids or to the N-or O-linked sugar chases of glycoproteins. The animal sialyltransferases are Golgi type II transmembrane glycosyltransferases. While the enzyme ST6Gal I uses the Galß1-4GlcNAc-R as the acceptor substrate which present in insects.
  Positive Control: Recombinant protein
  Cellular Location: Membrane
• Reconstitution and Storage: The antibodies are stable for 24 months from date of receipt when stored at -20C to -70C. The antibodies can be stored at 2C-8C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
• Immunogen: A synthetic peptide derived from the internal domain of Sialyltransferase protein from drosophila origin
• Purification: Affinity Purified
• Concentration: 100ug/200ul
• Specificity: ST3GAL4
• Formulation: This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
• Application Info: WB: 0.1-1 ug/ml | ELISA: 0.01-0.1 ug/ml | IP: 2-5 ug/ml | IHC: 2-10 ug/ml | FC: Not tested
• Reference: Anne Harduin-Lepers, et al. The animal sialyltransferases and sialyltransferase-related genes: a phylogenetic approach.
  Glycobiology 2005 15(8):805-817

Target Info

• Gene Symbol: SiaT
• Gene Full Name: Sialyltransferase
• Alias Symbols: alpha2,6-sialyltransferase;alpha-2,6-sialyltransferase;b-galactoside-a-2,6-sialyltransferase;CG4871;CG4871-PA;CG4871-PB;D.SialT;D.SiaT;Dm ST;Dmel\CG4871;Dmel_CG4871;DmPST;DmSialT;DSiaT;dST6Gal I;SialT;sialyltransferase;sialyltransferase-like;SiaT-PA;SiaT-PB;ST;ST6Gal.
• NCBI Gene Id: 37950
• Description of Target: Sialyltransferases are enzyme that transfer sialic acid to nascent oligosaccharide. Each silaytransferase is specific for a particular sμgar substRate. Sialytransferases add sialic acid to the terminal portins of the sialylated glycolipids or to the N-or O-linked sμgar chases of glycoproteins. The animal sialyltransferases are Golgi type II transmembrane glycosyltransferases.
  While the enzyme ST6Gal I uses the Galß1-4GlcNAc-R as the acceptor substRate which present in insects.
  
• Molecular Weight: 51