Anti-SOX6 Polyclonal Antibody (OAAI00495)

Catalog No: OAAI00495

Size:100UG

Western Blot: The whole tissue lysate derived from Mouse embryos was separated in 10% SDS-PAGE, transferred onto NC membrane, and immunoblotted by Rabbit anti – SOX6 antibody at 1:500 . An immunoreactive band around ~94 kDa was observed.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid. This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Flow cytometry|Immunohistochemistry|Immunoprecipitation|Western blot
• Additional Information: Background: Members of the SOX gene family encode proteins with homology to the HMG box DNA-binding domain of SRY, the Y-linked testis-determining gene. SOX genes are expressed during embryogenesis and are involved in the development of a wide range of different tissues. Mutations in SRY, SOX9 and SOX10 have been shown to be responsible for XY sex reversal, campomelic dysplasia and Waardenburg-Hirschsprung disease, respectively. It is likely that mutations in other SOX genes are responsible for a variety of human genetic diseases. SOX14 has been identified from a human genomic library and the mouse and chicken sequences obtained by polymerase chain reaction amplification. The SOX14 amino acid sequence is highly conserved across these species, suggesting an important role for this protein in vertebrate development. SOX14 is expressed in the neural tube and apical ectodermal ridge of the developing chicken limb. The SOX14 is associated to limb defects.
  Positive Control: Human glioma tissue
  Cellular Location: Nuclear
• Reconstitution and Storage: The antibodies are stable for 24 months from date of receipt when stored at -20C to -70C. The antibodies can be stored at 2C-8C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
• Immunogen: A synthetic peptide corresponding to the c-terminus (from 720AA-755aa) of human sox6 protein. This sequence is identical in human, mouse, rat, bovine, canis and chicken species.
• Purification: Affinity Purified
• Concentration: 100ug/200ul
• Specificity: SOX6
• Formulation: This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
• Application Info: WB: 0.1-1 ug/ml | ELISA: 0.01-0.1 ug/ml | IP: 2-5 ug/ml | IHC: 2-10 ug/ml | FC: Not tested
• Reference: Cohen-Barak,O., Hagiwara,N., Arlt,M.F., Horton,J.P. and Brilliant,M.H. Cloning, characterization and chromosome mapping of the Human SOX6 gene. Gene 265 (1-2), 157-164 (2001)

Target Info

• Gene Symbol: SOX6
• Gene Full Name: SRY-box transcription factor 6
• Alias Symbols: HSSOX6;SOXD;SRY (sex determining region Y)-box 6;SRY-box 6;SRY-box containing gene 6;TOLCAS;transcription factor SOX-6.
• NCBI Gene Id: 55553
• Description of Target: SOX6 gene encodes a member of the D subfamily of sex determining region y-related transcription factors that are characterized by a conserved DNA-binding domain termed the high mobility group box and by their ability to bind the minor groove of DNA. SOX6 protein is a transcriptional activator that is specifically expressed in the developing central nervous system and in the early stages of chondrogenesis in Mouse embryos. It is found to be essential to both cartilage formation/chondrogenesis and obesity-related insulin resistance. The SOX6 protein is also detected in glioma patient sera and brain tissues.
  
• Uniprot ID: P35712
• Molecular Weight: 90