Anti-ROCKII Antibody (Phospho-Thr 396) (OAAI00345)

Catalog No: OAAI00345

Size:100UG

Western Blot: The cell lysate derived from Conditioned 3T3 cell was immunoprecipitated by Rabbit anti Rho Kinase (pT396), (Cat#OAAI00345), then immunoprobed by the same antibody at 1:500 (lane 1). Lane 2 is a negative control.

Product Info

• Predicted Species Reactivity: Bovine|Canis|Chicken|Human|Mouse|Rat
• Product Format: Liquid. This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Flow cytometry|Immunohistochemistry|Immunoprecipitation|Western blot
• Additional Information: Background: Rho A-dependent kinase (ROCKII), or ROK-alpha, is identified a serine/threonine kinase, a downstream effector of the small GTPase Rho, mediating effects of Rho on the actin cytoskeleton, formation of actin stress fibers and focal adhesions, ROCKII alters cardiac myofilament response to Ca2+ by mechanisms involving phosphorylation of thin filament proteins. ROCK-II induces a depression in maximum ATPase rate and tension, which is associated with phosphorylation of troponin T (TnT), troponin I (TnI), and myosin-binding protein C (C-protein). The phosphorylation of Rho-kinase at Thr 249, Thr 396 plays important role in G-protein signaling..
  Positive Control: Conditioned 3T3 cel
• Reconstitution and Storage: The antibodies are stable for 24 months from date of receipt when stored at -20C to -70C. The antibodies can be stored at 2C-8C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
• Immunogen: A synthetic peptide corresponding to the epitope -VETFP- with a single phosphorylation site Thr396 of human RockII protein. This sequence is identical among human, mouse, rat, chicken, dog and bovine.
• Purification: Affinity Purified
• Concentration: 100ug/200ul
• Target Post-Translational Modification: Phosphorylated
• Specificity: ROCK2
• Formulation: This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
• Application Info: WB: 0.1-1 ug/ml | ELISA: 0.01-0.1 ug/ml | IP: 2-5 ug/ml | IHC: 2-10 ug/ml | FC: Not tested
• Reference: Susan Vahebi, et al, Functional Effects of Rho-Kinase–Dependent Phosphorylation of Specific Sites on Cardiac Troponin.
  Circulation Research. 2005;96:740-747.

Target Info

• Gene Symbol: ROCK2
• Gene Full Name: Rho associated coiled-coil containing protein kinase 2
• Alias Symbols: p164 ROCK-2;rho-associated protein kinase 2;rho-associated, coiled-coil-containing protein kinase II;ROCK-II.
• NCBI Gene Id: 9475
• Description of Target: Rho A-dependent kinase (ROCKII), or ROK-alpha, is identified a serine/threonine kinase, a downstream effector of the small GTPase Rho, mediating effects of Rho on the actin cytoskeleton, formation of actin stress fibers and focal adhesions, ROCKII alters cardiac myofilament response to Ca2+ by mechanisms involving phosphorylation of thin filament proteins.
  ROCK-II induces a depression in maximum ATPase Rate and tension, which is associated with phosphorylation of troponin T (TnT), troponin I (TnI), and myosin-binding protein C (C-protein). The phosphorylation of Rho-kinase at Thr 249, Thr 396 plays important role in G-protein signaling.
• Uniprot ID: O75116
• Molecular Weight: 165