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Catalog No: OAAI00735
Size:100UG
Price: $469.00
SKU
OAAI00735
Availability: Domestic: within 1-2 week delivery | International: 1-2 weeks
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Datasheets/ManualsPrintable datasheet for Anti-Rho-GDI Antibody (Phospho-Tyr156) (OAAI00735)
Product Info
Predicted Species ReactivityHuman|Mouse|Rat
Product FormatLiquid. This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
ClonalityPolyclonal
IsotypeRabbit IgG
HostRabbit
ApplicationEnzyme-linked immunosorbent assay|Flow cytometry|Immunohistochemistry|Immunoprecipitation|Western blot
Additional InformationBackground: Rho GTPases (Rac, Rho, and Cdc42) play important roles in regulating cell function through their ability to coordinate the actin cytoskeleton, modulate the formation of signaling reactive oxidant species, and control gene transcription. Activation of Rho GTPase signaling pathways requires the regulated release of Rho GTPases from Rho-GDI complexes, followed by their reuptake after membrane cycling. There are two isoforms, Rho-GDI alpha and Rho-GDI beta play synergistic roles in cell migration and development by modulating activation cycle of the Rho proteins. Src kinase binds and phosphorylates RhoGDI both in vitro and in vivo at Tyr156 which causes a dramatic decrease in the ability of RhoGDI to form a complex with RhoA, Rac1, or Cdc42. Src-mediated RhoGDI phosphorylation is a novel physiological mechanism for regulating Rho GTPase cytosol membrane–cycling and activity.
::Positive Control: MCF7
::Cellular Location: Cytoplasmic
Reconstitution and StorageThe antibodies are stable for 24 months from date of receipt when stored at -20C to -70C. The antibodies can be stored at 2C-8C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
ImmunogenA synthetic peptide surrounding the epitope - CRPEEYEFL- with a phosphorylation site at Tyrosine 156 of human Rho-GDI alpha protein. This sequence is identical among human, rat, mouse, bovine, chicken and dog.
PurificationAffinity Purified
Concentration100ug/200ul
Target Post-Translational ModificationPhosphorylated
SpecificityARHGDIA
FormulationThis affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
Application InfoWB: 0.1-1 ug/ml | ELISA: 0.01-0.1 ug/ml | IP: 2-5 ug/ml | IHC: 2-10 ug/ml | FC: Not tested
ReferenceIshizaki,H., et al. Defective chemokine-directed lymphocyte migRation and development in the absence of Rho guanosine diphosphate-dissociation inhibitors alpha and beta. J. Immunol. 177 (12), 8512-8521 (2006).
Celine DerMardirossian et al. Phosphorylation of RhoGDI by Src Regulates Rho GTPase Binding and Cytosol-Membrane Cycling. Mol. Biol. Cell. 17 (11). 4760-4768 (2006).
Description
Gene SymbolARHGDIA
Gene Full NameRho GDP dissociation inhibitor alpha
Alias Symbolsepididymis secretory sperm binding protein Li 47e;GDIA1;GDP-dissociation inhibitor, aplysia RAS-related 1;HEL-S-47e;NPHS8;Rho GDP dissociation inhibitor (GDI) alpha;rho GDP-dissociation inhibitor 1;RHOGDI;RHOGDI-1.
NCBI Gene Id396
Description of TargetRho GTPases (Rac, Rho, and Cdc42) play important roles in regulating cell function throμgh their ability to coordinate the actin cytoskeleton, modulate the formation of signaling reactive oxidant species, and control gene transcription. Activation of Rho GTPase signaling pathways requires the regulated release of Rho GTPases from Rho-GDI complexes, followed by their reuptake after membrane cycling. There are two isoforms, Rho-GDI alpha and Rho-GDI beta play synergistic roles in cell migRation and development by modulating activation cycle of the Rho proteins. Src kinase binds and phosphorylates RhoGDI both in vitro and in vivo at Tyr156 which causes a dramatic decrease in the ability of RhoGDI to form a complex with RhoA, Rac1, or Cdc42. Src-mediated RhoGDI phosphorylation is a novel physiological mechanism for regulating Rho GTPase cytosol membrane-cycling and activity.
Uniprot IDP52565
Molecular Weight23
  1. What is the species homology for "Anti-Rho-GDI Antibody (Phospho-Tyr156) (OAAI00735)"?

    The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".

  2. How long will it take to receive "Anti-Rho-GDI Antibody (Phospho-Tyr156) (OAAI00735)"?

    This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".

  3. What buffer format is "Anti-Rho-GDI Antibody (Phospho-Tyr156) (OAAI00735)" provided in?

    This item is provided in "Liquid. This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "Anti-Rho-GDI Antibody (Phospho-Tyr156) (OAAI00735)"?

    This target may also be called "epididymis secretory sperm binding protein Li 47e;GDIA1;GDP-dissociation inhibitor, aplysia RAS-related 1;HEL-S-47e;NPHS8;Rho GDP dissociation inhibitor (GDI) alpha;rho GDP-dissociation inhibitor 1;RHOGDI;RHOGDI-1." in publications.

  5. What is the shipping cost for "Anti-Rho-GDI Antibody (Phospho-Tyr156) (OAAI00735)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "Anti-Rho-GDI Antibody (Phospho-Tyr156) (OAAI00735)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "Anti-Rho-GDI Antibody (Phospho-Tyr156) (OAAI00735)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "23".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "Anti-Rho-GDI Antibody (Phospho-Tyr156) (OAAI00735)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.

  10. What are positive controls for "ARHGDIA"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "ARHGDIA"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "ARHGDIA"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "ARHGDIA"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "ARHGDIA"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "ARHGDIA"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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