Anti-Rho-GDI Antibody (Phospho-Tyr156) (OAAI00735)

Catalog No: OAAI00735

Size:100ug

Western Blot: The whole cell lysate derived from serum depleted MCF7 (Lane 1) and HELA (Lane 2) were separated in 12% SDS-PAGE and transferred onto NC membrane followed by immuoblotting of Rabbit anti-RhoGDI(pTyr156) (Cat#OAAI00735) at 1:500. An immunoreactive band at ~24 kDa was observed.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: IHC-P, WB
• Additional Information: Background: Rho GTPases (Rac, Rho, and Cdc42) play important roles in regulating cell function through their ability to coordinate the actin cytoskeleton, modulate the formation of signaling reactive oxidant species, and control gene transcription. Activation of Rho GTPase signaling pathways requires the regulated release of Rho GTPases from Rho-GDI complexes, followed by their reuptake after membrane cycling. There are two isoforms, Rho-GDI alpha and Rho-GDI beta play synergistic roles in cell migration and development by modulating activation cycle of the Rho proteins. Src kinase binds and phosphorylates RhoGDI both in vitro and in vivo at Tyr156 which causes a dramatic decrease in the ability of RhoGDI to form a complex with RhoA, Rac1, or Cdc42. Src-mediated RhoGDI phosphorylation is a novel physiological mechanism for regulating Rho GTPase cytosol membrane–cycling and activity.
  Positive Control: MCF7
  Cellular Location: Cytoplasmic
• Reconstitution and Storage: The antibodies are stable for 24 months from date of receipt when stored at -20C to -70C. The antibodies can be stored at 2C-8C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
• Immunogen: A synthetic peptide surrounding the epitope – CRPEEYEFL- with a phosphorylation site at Tyrosine 156 of human Rho-GDI alpha protein. This sequence is identical among human, rat, mouse, bovine, chicken and dog.
• Purification: The Rabbit IgG is purified by Epitope Affinity Purification
• Concentration: 100ug/200ul
• Target Post-Translational Modification: Phosphorylated
• Specificity: ARHGDIA
• Formulation: This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
• Application Info: WB: 0.1-1 ug/ml | ELISA: 0.01-0.1 ug/ml | IP: 2-5 ug/ml | IHC: 2-10 ug/ml | FC: Not tested
• Reference: Ishizaki,H., et al. Defective chemokine-directed lymphocyte migRation and development in the absence of Rho guanosine diphosphate-dissociation inhibitors alpha and beta. J. Immunol. 177 (12), 8512-8521 (2006).
  Celine DerMardirossian et al. Phosphorylation of RhoGDI by Src Regulates Rho GTPase Binding and Cytosol-Membrane Cycling. Mol. Biol. Cell. 17 (11). 4760-4768 (2006).
  

Target Info

• Gene Symbol: ARHGDIA
• Gene Full Name: Rho GDP dissociation inhibitor (GDI) alpha
• Alias Symbols: Rho GDP dissociation inhibitor (GDI) alpha, GDIA1, RHOGDI
• NCBI Gene Id: 396
• Description of Target: Rho GTPases (Rac, Rho, and Cdc42) play important roles in regulating cell function throμgh their ability to coordinate the actin cytoskeleton, modulate the formation of signaling reactive oxidant species, and control gene transcription. Activation of Rho GTPase signaling pathways requires the regulated release of Rho GTPases from Rho-GDI complexes, followed by their reuptake after membrane cycling. There are two isoforms, Rho-GDI alpha and Rho-GDI beta play synergistic roles in cell migRation and development by modulating activation cycle of the Rho proteins. Src kinase binds and phosphorylates RhoGDI both in vitro and in vivo at Tyr156 which causes a dramatic decrease in the ability of RhoGDI to form a complex with RhoA, Rac1, or Cdc42. Src-mediated RhoGDI phosphorylation is a novel physiological mechanism for regulating Rho GTPase cytosol membrane-cycling and activity.
  
• Uniprot ID: P52565
• Molecular Weight: 23 kDa