Anti-Retinoic Acid Receptor, Alpha-Isotype (OAPC00018)

Catalog No: OAPC00018 (Formerly GWB-5E8CCA)

Size:100 ul

Western blot of rat hippocampal lysate showing specific immunolabeling of the ~48k RAR- alpha protein.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig
• Product Format: Liquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Clonality: Monoclonal
• Clone: 763
• Isotype: IgG¹
• Host: Mouse
• Application: WB
• Additional Information: Packaging :
  100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Reconstitution and Storage: Store at -20C; stable for at least 1 year.
• Immunogen: Peptide corresponding to amino acid residues from the N-terminal region of human retinoic acid receptor, alpha -isotype.
• Purification: Prepared from mouse ascites by ammonium sulfate precipitation followed affinity purification on a Protein G column.
• Predicted Homology Based on Immunogen Sequence: Cow; Dog; Guinea Pig; Human; Mouse; Rat
• Specificity: Specific for the ~48k RAR- alpha isotype.
• Application Info: The antibody has been directly tested for reactivity in Western blots in rat and human tissues. It is anticipated that the antibody will also work with bovine, canine, mouse and guinea pig tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
  Western blot of rat hippocampal lysate showing specific immunolabeling of the ~48k RAR- alpha protein.
  
• Reference: Abu J, Batuwangala M, Herbert K, Symonds P (2005) Retinoic acid and retinoid receptors: potential chemopreventive and therapeutic role in cervical cancer. Lancet Oncol 6:712-720.
  Joshi S, Guleria R, Pan J, Dipette D, Singh US (2005) Retinoic acid receptors and tissue-trans-glutaminase mediate short-term effect of retinoic acid on migration and invasion of neuroblastoma SH-SY5Y cells. Oncogene advance online publication 12 September 2005; doi: 10.1038/sj.onc.1209027.
  Lane MA, Bailey SJ (2005) Role of retinoid signalling in the adult brain. Prog Neurobiol 75:275-293.

Target Info

• Gene Symbol: RARA
• Alias Symbols: RAR, NR1B1
• NCBI Gene Id: 5914
• Protein Name: Retinoic acid receptor alpha
• Description of Target: Retinoic acid (RA; active metabolite of vitamin A) plays a prominent role in regulating the transition of proliferating precursor cells (such as carcinoma cells and neuronal precursors) to postmitotic differentiated cells (Joshi et al., 2005). The retinoid X receptors (RXRs) family (RXR alpha, beta and gamma), preferentially bind 9-cis-RA and regulate gene transcription by forming heterodimers with a second family of RA receptors. RAs have been suggested to potentially play a therapeutic role in cervical cancer (Abu et al., 2005). RAs are known to play key roles in neuronal development and an increasing body of evidence indicates that retinoid signaling may regulate synaptic plasticity and associated learning and memory behaviors (Lane and Bailey, 2005).
• Uniprot ID: P10276
• Protein Accession #: NP_000955
• Nucleotide Accession #: NM_000964
• Molecular Weight: ~48kDa