Anti-RAIDD (human) (AT100) Antibody (OAED00256)

Catalog No: OAED00256

Size:50 ug

Figure: Detection of endogenous RAIDD (human) in HEK293T cells by the PAb to RAIDD (AT100). Method: Total cell extracts (50 ug) were run on a SDS-gel, and revealed with anti-RAIDD antibody (AT100, 1:1000, affinity purified antibody, in 5% milk powder) for 1 hour at RT followed by incubation with HRP-coupled anti-rabbit secondary antibody (1:4’000). The arrow indicates endogenous human RAIDD.

Product Info

• Predicted Species Reactivity: Human
• Clonality: Polyclonal
• Host: Rabbit
• Application: IP, WB
• Reconstitution and Storage: Long Term Storage : -20C
  Short Term Storage : +4C
  Handling : Avoid freeze/thaw cycles.
  
• Immunogen: Recombinant human RAIDD (aa 1-199).
• Concentration: 1mg/ml
• Specificity: Recognizes human RAIDD.
• Formulation: Liquid. In PBS containing 0.02% sodium azide.
• Purity: Affinity purified.
  
• Application Info: Immunoprecipitation
  Western Blot (1:1,000)
• Reference: RAIDD aggregation facilitates apoptotic death of PC12 cells and sympathetic neurons: O. Jabado, et al.; Cell Death Differ. 11, 618 (2004), Abstract;
  The PIDDosome, a protein complex implicated in activation of caspase-2 in response to genotoxic stress: A. Tinel and J. Tschopp; Science 304, 843 (2004), Abstract;

Target Info

• Gene Symbol: CRADD
• Alias Symbols: MRT34, RAIDD
• NCBI Gene Id: 8738
• Protein Name: Death domain-containing protein CRADD
• Description of Target: RAIDD (RIP-associated ICH-1 homologous protein with a death domain) is an adaptor molecule that mediates the action of cysteine proteases involved in apoptosis. In human cell lines, the caspase-2 adaptor RAIDD interacts selectively with caspase-2 through its caspase recrui™ent domain (CARD) and leads to caspase-2-dependent death. RAIDD overexpression induced caspase-2, CARD- and caspase-9-dependent apoptosis of PC12 cells and sympathetic neurons. Apoptosis correlated with the formation of discrete perinuclear aggregates. Both death and aggregates required the expression of full-length RAIDD. It has been reported that activation of caspase-2 occurs in a complex that contains the death domain–containing protein PIDD, whose expression is induced by p53, and the adaptor protein RAIDD.
• Uniprot ID: P78560