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Anti-Rabbit IgG Antibody (OAPB01378)

  • Catalog#: OAPB01378
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2 mg
$310.00
Discontinued
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Conjugation Options

OAPB01378-FITC Conjugated

OAPB01378-HRP Conjugated

OAPB01378-Biotin Conjugated

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Host:
Goat
Purification:
Peroxidase Conjugated Affinity Purified anti-Rabbit IgG was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by
Application:
ELISA, WB, IHC
Immunogen:
Rabbit IgG whole molecule
Predicted Species Reactivity:
Rabbit
Predicted Homology Based on Immunogen Sequence:
Rabbit
Reconstitution and Storage:
Peroxidase Conjugated Affinity Purified anti-Rabbit IgG can be stored vial at 4C prior to restoration. Restore with 1.0 ml of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
Datasheets/Manuals:
Printable datasheet for OAPB01378
Conjugation:
Horseradish Peroxidase (HRP)
Application Info:
Peroxidase Conjugated Affinity Purified anti-Rabbit IgG is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended dilutions are as follows, ELISA 1:20,000 - 1:100,000, Western Blot 1:2,000 - 1:10,000, Immunohistochemistry 1:1,000 - 1:5,000. (Optimal dilution should be determined by user.)
Additional Information:
Preparation: PHYSICAL STATE: Lyophilized ANTIBODY CONCENTRATION: 2.0 mg/ml (by UV absorbance at 280 nm)
:::
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Publications:

Sassek, M; Pruszynska-Oszmalek, E; Kołodziejski, PA; Szczepankiewicz, D; Kaczmarek, P; Wieloch, M; Kurto, K; Nogowski, L; Nowak, KW; Strowski, MZ; Mackowiak, P; Resistin is produced by rat pancreatic islets and regulates insulin and glucagon in vitro secretion. 8, 177-185 (2016). ELISA, WB, IHC, Rabbit 27797297

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