Anti-PKC-epsilon Polyclonal Antibody (OAAI00203)

Catalog No: OAAI00203

Size:100UG

WB: The cell lysate derived from SKBR-3 was immunoblotted by the Rabbit anti PKC-epsilon (Cat#OAAI00203) @ 1:500. An immunoreactive bands was observed around ~85 kDa.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid. This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Flow cytometry|Immunohistochemistry|Immunoprecipitation|Western blot
• Additional Information: Background: Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. Protein kinase C epsilon is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, insulin exocytosis, It is physically associated with the phosphorylation of cytokeratins 8/18.
  Positive Control: SKBR-3 cells
  Cellular Location: Cytoplasmic
• Reconstitution and Storage: The antibodies are stable for 24 months from date of receipt when stored at -20C to -70C. The antibodies can be stored at 2C-8C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
• Immunogen: A synthetic peptide corresponding to C-term of human PKC epsilon. This sequence is identical to mouse and rat.
• Purification: Affinity Purified
• Concentration: 100ug/200ul
• Specificity: PRKCE
• Formulation: This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
• Application Info: WB: 0.1-1 ug/ml | ELISA: 0.01-0.1 ug/ml | IP: 2-5 ug/ml | IHC: 2-10 ug/ml | FC: Not tested
• Reference: Omary,M.B., Baxter,G.T., Chou,C.F., Riopel,C.L., Lin,W.Y. and Strulovici,B. PKC epsilon-related kinase associates with and phosphorylates cytokeRatin 8 and 18. J. Cell Biol. 117 (3), 583-593 (1992)

Target Info

• Gene Symbol: PRKCE
• Gene Full Name: protein kinase C epsilon
• Alias Symbols: nPKC-epsilon;PKCE;protein kinase C epsilon type.
• NCBI Gene Id: 5581
• Description of Target: Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. Protein kinase C epsilon is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, insulin exocytosis, It is physically associated with the phosphorylation of cytokeRatins 8/18.
  
• Uniprot ID: Q02156
• Molecular Weight: 80-85