Anti-Peripherin (OAPC00054)

Catalog No: OAPC00054

Size:100 ul

Western blot of rat cerebellar lysate showing specific immunolabeling of the ~ 57k peripherin protein.

Immunostaining of cultured newborn rat neurons and glia showing peripherin in green and neurofilament L in red. Western blot of rat cerebellar lysate showing specific immunolabeling of the ~ 57k peripherin protein.

Product Info

• Predicted Species Reactivity: Most mammals
• Product Format: Liquid.
• Clonality: Monoclonal
• Clone: 7C5
• Isotype: IgG₁
• Host: Mouse
• Application: WB, IF
• Reconstitution and Storage: Store at -207deg;C in undiluted aliquots; stable for at least 1 year. Avoid freeze/thaw cycles.
• Immunogen: Recombinant rat peripherin expressed in and purified from E. coli.
• Purification: Unpurified, concentrated culture supernatant.
• Predicted Homology Based on Immunogen Sequence: Most mammals
• Specificity: Specific for the ~57kDa peripherin protein. This antibody performs well on aldehyde fixed tissues.
• Application Info: The antibody has been directly tested for reactivity in many mammalian tissues.
  Recommended Dilutions:
  WB: 1:1000
  IF: 1:200
• Reference: 1. Terao E, Janssens S, van den Bosch de Aquilar P, Portier M, Klosen P (2000) In vivo expression of the intermediate filament peripherin in rat motorneurons: modulation by inhibitory and stimulatory signals. Neuroscience 101(3):679-88.
  2.Roberson J, Doroudchi MM, Nguyen MD, Durham, HD, Shaw G, Julien JP, Mushynski WE. (2003) A neurotoxic peripherin splice variant in a mouse model of ALS. J. Cell Biol. 160(6):939-49.
  3.Boitard C, Villa MC, Becourt C, Gia HP, Huc C, Sempe P, Portier MM, Bach JF (1992)
  Peripherin: an islet antigen that is cross-reactive with nonobese diabetic mouse class II gene products. Proc Natl Acad Sci USA 89(1):172-6.

Target Info

• Gene Symbol: PRPH
• NCBI Gene Id: 3414
• Protein Name: Peripherin
• Description of Target: Peripherin is a ~57kDa intermediate filament subunit found initially in sensory neurons of the peripheral nervous systems, which gives the protein its name. Subsequently, peripherin was found in some sensory and other neurons of the central nervous system and also in PC12 cells. Peripherin is also expressed in certain neuroendocrine tumors and in the insulin producing cells of the pancreas. Peripherin belongs to the Class III family of intermediate filament subunits which also includes vimentin, glial fibrillary acidic protein (GFAP) and desmin. In contrast to the neurofilaments, peripherin is strongly up-regulated after nerve injury (1). Antibodies to peripherin can be used in identifying, classifying, and studying neurons throughout the nervous system. Peripherin is also a good diagnostic marker for ballooned axons seen in Lou Gehrig's disease (Amyotrophic lateral sclerosis) and some neuronally derived tumors (2). Autoantibodies to peripherin are frequently seen in the sera of patients with diabetes (3). Peripherin is not related to peripherin/RDS, a protein of the photoreceptor outer membrane mutations of which are causative of certain forms of slow retinal degeneration.
• Uniprot ID: P21807
• Molecular Weight: ~57kDa