Anti-P53 Monoclonal Antibody (OAAI00285)

Catalog No: OAAI00285

Size:100UG

IHC: The human breast cancer tissue stained with Mouse anti P53 monoclonal antibody (Cat#OAAI00285) at 1:500. (Note: formalin -fixed, paraffin -embedded sections need 15 minutes heat - induced epitope retrieval in 10 mM citrate buffer, pH 6.0, and 30 minutes incubation at room temperature with the primary antibody.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid. This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
• Clonality: Monoclonal
• Clone: DO-7
• Isotype: IgG2a
• Host: Mouse
• Application: Enzyme-linked immunosorbent assay|Flow cytometry|Immunohistochemistry|Immunoprecipitation|Western blot
• Additional Information: Background: P53, also known as protein 53, or tumor suppressor protein 53, plays important roles in cellular response to DNA damage and other genomic aberration. Mutations in the p53 tumor suppressor gene giving rise to mutant p53 proteins are among the most common genetic alterations associated with tumor cells. P53 protein is phosphorylated at multiple sites in vivo and in vitro in response to different stimuli. UV caused DNA damage can induce Ser9, Ser15, Ser20 phosphorylation while ATM, ATR, DNA-PK at Ser15 and Ser37.
  Positive Control: Breast and colon carcinomas
  Cellular Location: Nuclear
• Reconstitution and Storage: The antibodies are stable for 24 months from date of receipt when stored at -20C to -70C. The antibodies can be stored at 2C-8C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
• Immunogen: The recombinant protein of human P53 protein.
• Purification: The mouse IgG is purified by Protein A-Affinity Chromatography according to Isotyping
• Concentration: 100ug/200ul
• Specificity: TP53
• Formulation: This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
• Application Info: WB: 0.1-1 ug/ml | ELISA: 0.01-0.1 ug/ml | IP: 2-5 ug/ml | IHC: 2-10 ug/ml | FC: Not tested
• Reference: Zakut-Houri,R., Bienz-Tadmor,B., Givol,D. and Oren,M. Human p53 cellular tumor antigen: cDNA sequence and expression in COS cells. EMBO J. 4 (5), 1251-1255 (1985)
• Description:
  View product citations for Anti-P53 Monoclonal Antibody (OAAI00285) on CiteAb

Target Info

• Gene Symbol: TP53
• Gene Full Name: tumor protein p53
• Alias Symbols: antigen NY-CO-13;BCC7;BMFS5;cellular tumor antigen p53;LFS1;mutant tumor protein 53;P53;p53 tumor suppressor;phosphoprotein p53;transformation-related protein 53;TRP53;tumor protein 53;tumor supressor p53.
• NCBI Gene Id: 7157
• Description of Target: P53, also known as protein 53, or tumor suppressor protein 53, plays important roles in cellular response to DNA damage and other genomic aberRation. Mutations in the p53 tumor suppressor gene giving rise to mutant p53 proteins are among the most common genetic alteRations associated with tumor cells. P53 protein is phosphorylated at multiple sites in vivo and in vitro in response to different stimuli. UV caused DNA damage can induce Ser9, Ser15, Ser20 phosphorylation while ATM, ATR, DNA-PK at Ser15 and Ser37.
  
• Uniprot ID: P04637
• Molecular Weight: 53