Anti-P53 Antibody (Phospho-Ser37) (OAAI00469)

Catalog No: OAAI00469

Size:100UG

WB: The cell lysate derived from UV stimulated Hela cells was immunoprobed by Rabbit anti-p53 (pS37) (cat#OAAI00469) at 1:500. An immunoreactive band is observed around ~53kDa

IHC: UV treated Hela cells cultured in the cell chamber, fixed, and immunoprobed by Rabbit Anti-p53 (pS37) antibody, (Cat#OAAI00469) at 1:100 for 10 min @ RT.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid. This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Flow cytometry|Immunohistochemistry|Immunoprecipitation|Western blot
• Additional Information: Background: P53, also known as protein 53, or tumor suppressor protein 53, plays important roles in cellular response to DNA damage and other genomic aberration. Mutations in the p53 tumor suppressor gene giving rise to mutant p53 proteins are among the most common genetic alterations associated with tumor cells. P53 protein is phosphrylated at multiple sites in vivo and in vitro in response to different stimuli. UV caused DNA damage can induce Ser9, Ser15, Ser20 phosphorylation while ATM, ATR, DNA-PK at Ser15 and Ser37.
  Positive Control: UV treated Hela cells, Breast carcinoma
  Cellular Location: Nuclear
• Reconstitution and Storage: The antibodies are stable for 24 months from date of receipt when stored at -20C to -70C. The antibodies can be stored at 2C-8C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
• Immunogen: A synthetic peptide corresponding to the epitope -LPSPH- at a phosphorylation site at serine 37 of human p53 protein
• Purification: Affinity Purified
• Concentration: 100ug/200ul
• Target Post-Translational Modification: Phosphorylated
• Specificity: TP53
• Formulation: This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
• Application Info: WB: 0.1-1 ug/ml | ELISA: 0.01-0.1 ug/ml | IP: 2-5 ug/ml | IHC: 2-10 ug/ml | FC: Not tested
• Reference: Sylvie Labrecque, et al. Analysis of the Anti-p53 Antibody Response in Cancer Patients (1993) Cancer Research 53, 3468-3471

Target Info

• Gene Symbol: TP53
• Gene Full Name: tumor protein p53
• Alias Symbols: antigen NY-CO-13;BCC7;BMFS5;cellular tumor antigen p53;LFS1;mutant tumor protein 53;P53;p53 tumor suppressor;phosphoprotein p53;transformation-related protein 53;TRP53;tumor protein 53;tumor supressor p53.
• NCBI Gene Id: 7157
• Description of Target: P53, also known as protein 53, or tumor suppressor protein 53, plays important roles in cellular response to DNA damage and other genomic aberRation. Mutations in the p53 tumor suppressor gene giving rise to mutant p53 proteins are among the most common genetic alteRations associated with tumor cells. P53 protein is phosphrylated at multiple sites in vivo and in vitro in response to different stimuli. UV caused DNA damage can induce Ser9, Ser15, Ser20 phosphorylation while ATM, ATR, DNA-PK at Ser15 and Ser37.
  
• Uniprot ID: P04637
• Molecular Weight: 53