Anti-P19/INK4d Polyclonal Antibody (OAAI00323)

Catalog No: OAAI00323

Size:100UG

Western Blot: The tissue lysate derived from mouse liver was immunoblotted by Rabbit anti p19/INK4d (Cat#OAAI00323) antibody at 1:500. An immunoreactive band was observed around 19 kDa

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid. This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Flow cytometry|Immunohistochemistry|Immunoprecipitation|Western blot
• Additional Information: Background: The p19 INK4d, a member of the INK4 family of cyclin-dependent kinase (CDK) inhibitors, plays a dual role in the DNA damage response, inhibiting cell proliferation and promoting DNA repair. This protein has been shown to form a stable complex with CDK4 or CDK6, and prevent the activation of the CDK kinases, thus function as a cell growth regulator that controls cell cycle G1 progression. The abundance of the transcript of this gene was found to oscillate in a cell-cycle dependent manner with the lowest expression at mid G1 and a maximal expression during S phase. The negative regulation of the cell cycle involved in this protein was shown to participate in repressing neuronal proliferation, as well as spermatogenesis. The p19INK4d has been reported to become upregulated in response to UV irradiation and a great variety of genotoxic agents.
  Positive Control: Mouse liver
  Cellular Location: Nuclear
• Reconstitution and Storage: The antibodies are stable for 24 months from date of receipt when stored at -20C to -70C. The antibodies can be stored at 2C-8C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
• Immunogen: A synthetic peptide surrounding to the C-terminus of p19 protein from human. This sequence is identical to human, mouse and rat origins.
• Purification: Affinity Purified
• Concentration: 100ug/200ul
• Specificity: CDKN2D
• Formulation: This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
• Application Info: WB: 0.1-1 ug/ml | ELISA: 0.01-0.1 ug/ml | IP: 2-5 ug/ml | IHC: 2-10 ug/ml | FC: Not tested
• Reference: Chan,F.K., Zhang,J., Cheng,L., Shapiro,D.N. and Winoto,A. Identification of Human and Mouse p19, a novel CDK4 and CDK6 inhibitor with homology to p16ink4. Mol. Cell. Biol. 15 (5), 2682-2688 (1995)

Target Info

• Gene Symbol: CDKN2D
• Gene Full Name: cyclin dependent kinase inhibitor 2D
• Alias Symbols: CDK inhibitor p19INK4d;cell cycle inhibitor, Nur77 associating protein;cyclin-dependent kinase 4 inhibitor D;cyclin-dependent kinase 4 inhibitor D p19;cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4);inhibitor of cyclin-dependent kinase 4d;INK4D;p19;p19-INK4D.
• NCBI Gene Id: 1032
• Description of Target: The p19 INK4d, a member of the INK4 family of cyclin-dependent kinase (CDK) inhibitors, plays a dual role in the DNA damage response, inhibiting cell prolifeRation and promoting DNA repair. This protein has been shown to form a stable complex with CDK4 or CDK6, and prevent the activation of the CDK kinases, thus function as a cell growth regulator that controls cell cycle G1 progression. The abundance of the transcript of this gene was found to oscillate in a cell-cycle dependent manner with the lowest expression at mid G1 and a maximal expression during S phase. The negative regulation of the cell cycle involved in this protein was shown to participate in repressing neuronal prolifeRation, as well as spermatogenesis. The p19INK4d has been reported to become upregulated in response to UV irradiation and a great variety of genotoxic agents.
  
• Uniprot ID: P55273
• Molecular Weight: 19