Anti-NF-kB p105/p50 Antibody (Phospho-S337) (OAAI00072)

Catalog No: OAAI00072

Size:100UG

Product Info

• Predicted Species Reactivity: Human|Mouse
• Product Format: Liquid. This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Flow cytometry|Immunohistochemistry|Immunoprecipitation|Western blot
• Additional Information: Background: NFkB is a heterodimer that consists of a 50 kDa DNA binding subunit (p50/NFkB1)) and a 65 kDa transactivation subunit (p65/RelA). Both of these subunits exhibit sequence homology to the proto-oncogene c-Rel. The p50 has an isoform called p49/p52, and both proteins are derived from the amino-terminal of precursor protein p105 and p100. The p50/p65 heterodimer remains in the cytosol in an inactive form as a complex with its inhibitor, IkB. Upon stimulation of cells by a wide variety of stimuli such as lipopolysaccharide (LPS), pro-inflammatory cytokines (IL-1 & TNF, etc.), and viral infection, IkB is phosphorylated and degraded by proteosome. The active NFkB heterodimer is translocated into the nucleus and induces gene expression.The inhibition of p53 activity is dependent upon phosphorylation of p65 (RelA) at S536 by the upstream kinase IKK beta.
  Positive Control: all human tissuse
  Cellular Location: Nucleus. Cytoplasm.
• Reconstitution and Storage: The antibodies are stable for 24 months from date of receipt when stored at -20C to -70C. The antibodies can be stored at 2C-8C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
• Immunogen: A synthetic peptide corresponding to the epitope containing a phosphorylation site 337 human NFkB/p105 protein. This sequence is identical within human and mouse.
• Purification: Affinity Purified
• Concentration: 100ug/200ul
• Target Post-Translational Modification: Phosphorylated
• Specificity: NFKB
• Formulation: This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
• Application Info: WB: 0.1-1 ug/ml | ELISA: 0.01-0.1 ug/ml | IP: 2-5 ug/ml | IHC: 2-10 ug/ml | FC: Not tested
• Publications:

  Crucial role of OX40/OX40L signaling in a murine model of asthma. Mol Med Rep. 17, 4213-4220 (2018). 29344664

• Description:
  View product citations for Anti-NF-kB p105/p50 Antibody (Phospho-S337) (OAAI00072) on CiteAb

Target Info

• Gene Symbol: NFKB1
• Gene Full Name: nuclear factor kappa B subunit 1
• Alias Symbols: CVID12;DNA-binding factor KBF1;EBP-1;KBF1;NFkappaB;NF-kappaB;NF-kappa-B1;NF-kappabeta;NF-kB;NF-kB1;NFKB-p105;NFKB-p50;nuclear factor kappa-B DNA binding subunit;nuclear factor NF-kappa-B p105 subunit;nuclear factor NF-kappa-B p50 subunit;nuclear factor of kappa light polypeptide gene enhancer in B-cells 1.
• NCBI Gene Id: 4790
• Description of Target: NfkB is a heterodimer that consists of a 50 kDA DNA binding subunit (p50/NFkB1) and a 65 kDa transactivation subnit (p65/ReIA). Both of these subunits exhibit sequence homology to the proto-oncogene c-Rel. The NF-kB1 gene encodes a 105 kDa protein which can undergo cotranslational processing by teh 26S proteasome to produce a 50 kDa protein. The 105 kDa protein is a Rel protein-specific transcription inhibitor and the 50 kDa protein a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth.
• Uniprot ID: P19838
• Molecular Weight: 105&50