Anti-Neurofilament M (NF-M) (OAPC00029)

Catalog No: OAPC00029

Size:100 ul

Western blot of rat cortex lysate showing specific immunolabeling of the ~ 145k NF-M protein.

Immunostaining of cultured rat neurons and glia showing labeling of NF-M in red.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid containing 10 mM sodium azide.
• Clonality: Polyclonal
• Host: Chicken
• Application: WB, IF
• Additional Information: Packaging :
  Liquid containing 10mM sodium azide.
• Reconstitution and Storage: Store at -20C in undiluted aliquots; stable for at least 1 year. Avoid freeze/thaw cycles.
• Immunogen: Recombinant fusion protein containing the extreme C-terminus of rat NF-M expressed in and purified from E. coli.
• Purification: Total IgY fraction.
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Specificity: Specific for the ~145k Neurofilament M protein.
• Application Info: The antibody has been directly tested for reactivity in chicken, human, mouse and rat tissues.
  Immunostaining of cultured rat neurons and glia showing labeling of NF-M in red.
  Western blot of rat cortex lysate showing specific immunolabeling of the ~ 145k NF-M protein.
• Reference: 1.Harris, J., Ayyub, C. and Shaw G. (1991) A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H. J Neurosci Res 30:47-62.
  2.
  Hu YY, He SS, Wang XC, Duan QH, Khatoon S, Igbal K, Grundke-Igbal I, Wang JZ (2002) Elevated levels of phosphorylated neurofilament proteins in cerebrospinal fluid of Alzheimer disease patients. Neurosci Lett 320(3):156-60.

Target Info

• Gene Symbol: NEFM
• Alias Symbols: Nfm, Nef3
• NCBI Gene Id: 24588
• Protein Name: Neurofilament medium polypeptide
• Description of Target: Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H (1). NF-M is the neurofilament middle or medium molecular weight polypeptide and runs on SDS-PAGE gels at 145-160 kDa, with some variability across species boundaries. Antibodies to NF-M are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-M antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) and Alzheimer's disease (2).
• Uniprot ID: P12839
• Protein Accession #: NP_058725.1
• Molecular Weight: ~145kDa