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- $55: Antibody & Protein in US
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- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for OAPC00025 |
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Predicted Species Reactivity | Human, Mouse, Rat, Chicken |
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Product Format | Liquid 10mM sodium azide. Adequate amount of material to conduct 10 mini Western blots. |
Clonality | Polyclonal |
Host | Chicken |
Application | WB, IF |
Reconstitution and Storage | Store at -20°C in undiluted aliquots; stable for at least 1 year. Avoid freeze/thaw cycles. |
Immunogen | Purified bovine NF-H |
Purification | Total IgY fraction |
Concentration | Varies by lot. See vial for concentration |
Specificity | Specific for the ~200k Neurofilament H protein in Western Blots of chicken, human, mouse and rat tissues. |
Intended Use | For research use only |
Application Info | The antibody has been directly tested for reactivity in chicken, mouse, human, and rat tissues. |
Reference | 1.Harris, J., Ayyub, C. and Shaw G. (1991) A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H. J Neurosci Res 30:47-62. 2. Mendonca DM, Chimelli L, Martinez AM. (2005) Quantitative evidence for neurofilament heavy subunit aggregation in motor neurons of spinal cords of patients with amyotrophic lateral sclerosis. Braz J Med Biol Res. 38(6):925-933. 3.Hu YY, He SS, Wang XC, Duan QH, Khatoon S, Igbal K, Grundke-Igbal I, Wang JZ (2002) Elevated levels of phosphorylated neurofilament proteins in cerebrospinal fluid of Alzheimer disease patients. Neurosci Lett 320(3):156-60. |
Gene Symbol | NEFH |
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Gene Full Name | neurofilament, heavy polypeptide |
Alias Symbols | NFH, CMT2CC |
NCBI Gene Id | 4744 |
Protein Name | Neurofilament heavy polypeptide |
Description of Target | Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H (1). NF-H is the neurofilament high or heavy molecular weight polypeptide and runs on SDS-PAGE gels at 200-220 kDa, with some variability across species boundaries. Antibodies to NF-H are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-H antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) (2) and Alzheimer's disease (3). |
Uniprot ID | P12036 |
Protein Accession # | NP_066554 |
Nucleotide Accession # | NM_021076 |
Molecular Weight | ~200kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Anti-Neurofilament H (NF-H) (OAPC00025)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat, Chicken".
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How long will it take to receive "Anti-Neurofilament H (NF-H) (OAPC00025)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "Anti-Neurofilament H (NF-H) (OAPC00025)" provided in?
This item is provided in "Liquid 10mM sodium azide. Adequate amount of material to conduct 10 mini Western blots.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Anti-Neurofilament H (NF-H) (OAPC00025)"?
This target may also be called "NFH, CMT2CC" in publications.
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What is the shipping cost for "Anti-Neurofilament H (NF-H) (OAPC00025)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Anti-Neurofilament H (NF-H) (OAPC00025)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Anti-Neurofilament H (NF-H) (OAPC00025)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "~200kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Anti-Neurofilament H (NF-H) (OAPC00025)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "NEFH"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "NEFH"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "NEFH"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "NEFH"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "NEFH"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "NEFH"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.