Anti-Microtubule Associated Protein 2 (MAP2) (OAPC00006)

Catalog No: OAPC00006

Size:100 ul

Western blot of rat cortex lysate showing specific immunolabeling of the ~280k MAP2 protein.

Mixed neuron/glial cultures. The perikarya and dendrites of neurons are strongly and specifically stained with the MAP2 antibody (red). Cell nuclei are visualized with DAPI DNA stain.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Cow
• Product Format: Liquid. containing 10 mM NaN₃. Adequate amount of material to conduct 10 mini-Western Blots.
• Clonality: Polyclonal
• Host: Chicken
• Application: WB, IF
• Reconstitution and Storage: Store at -20°C; stable for one year. Avoid freeze/thaw cycles.
• Purification: The antigen is a recombinant bovine MAP2 expressed in and purified from E. coli. The antibody is total IgY fractionTotal IgY fraction
• Concentration: Varies by lot. See vial for concentration.
• Specificity: Specific for the ~ 280k MAP2 protein in Western blots of rat brain homogenate.
• Application Info: WB: 1:20,000
  IF: 1:2,500
  Optimal dilutions should be determined by the end user.

Target Info

• Gene Symbol: MAP2
• Alias Symbols: MAP-2, MAP2A, MAP2B, MAP2C
• NCBI Gene Id: 4133
• Protein Name: Microtubule-associated protein 2
• Description of Target: Microtubules are 25nm diameter protein rods found in most kinds of eukaryotic cells. They are polymerized from a dimeric subunit made of one a subunit and one b tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein tau (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5 (Kindler & Gardner 1994). MAP2 is made up of two ~280kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein tau (tau). MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. MAP2 has been recently shown to be the specific receptor for the neurosteroid pregnenolone.
• Uniprot ID: P11137
• Protein Accession #: NP_002365
• Nucleotide Accession #: NM_001039538
• Molecular Weight: ~280kDa