Anti-JNK/SAPK Antibody (Phospho-Thr183/pTyr185) (OAAI00398)

Catalog No: OAAI00398

Size:100UG

Dot Blot: 1 ?g peptide was blot onto NC membrane A: JNK(pT183, pY185) B: JNK (Non phosphospeicfic) C: Non-related Phosphopeptide) were blotted at a 1:2000 dilution by: 1: Rabbit anti- JNK(pT183/pY185), cat#OAAI00398).

Product Info

• Predicted Species Reactivity: Human|Rat
• Product Format: Liquid. This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Flow cytometry|Immunohistochemistry|Immunoprecipitation|Western blot
• Additional Information: Background: c-Jun N-terminal Kinase (JNK) is a Stress Activated Protein Kinase/Map Kinase (SAPK/MAPK). There are three isoforms, JNK1 (~49 kDa), JNK2 (~55 kDa), and JNK3 (~54 kDa). JNK is dually phosphorylated at Thr 183 and Tyr 185 by protein kinases MKK4 and MKK7. The JNK and p38 pathways function to modulate cell-cycle, apoptotic and transcriptional responses to stress.
  Positive Control: UV treated-HEK293
  Cellular Location: Cytoplasm
• Reconstitution and Storage: The antibodies are stable for 24 months from date of receipt when stored at -20C to -70C. The antibodies can be stored at 2C-8C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
• Immunogen: A synthetic peptide surrounding to the epitope -TPY- with dual phosphorylation sites at Thr183 and Tyr185 of human JNK/SAPK .
• Purification: Affinity Purified
• Concentration: 100ug/200ul
• Target Post-Translational Modification: Phosphorylated
• Specificity: MAPK8
• Formulation: This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
• Application Info: WB: 0.1-1 ug/ml | ELISA: 0.01-0.1 ug/ml | IP: 2-5 ug/ml | IHC: 2-10 ug/ml | FC: Not tested
• Reference: Yogev,O., et al. DNA damage-dependent translocation of B23 and p19 ARF is regulated by the Jun N-terminal kinase pathway. Cancer Res. 68 (5), 1398-1406 (2008)

Target Info

• Gene Symbol: MAPK8
• Gene Full Name: mitogen-activated protein kinase 8
• Alias Symbols: c-Jun N-terminal kinase 1;JNK;JNK1;JNK1A2;JNK21B1/2;JNK-46;JUN N-terminal kinase;MAP kinase 8;mitogen-activated protein kinase 8;PRKM8;SAPK1;SAPK1c;stress-activated protein kinase 1;stress-activated protein kinase 1c.
• NCBI Gene Id: 5599
• Description of Target: c-Jun N-terminal Kinase (JNK) is a Stress Activated Protein Kinase/Map Kinase (SAPK/MAPK). There are three isoforms, JNK1 (~49 kDa), JNK2 (~55 kDa), and JNK3 (~54 kDa). JNK is dually phosphorylated at Thr 183 and Tyr 185 by protein kinases MKK4 and MKK7. The JNK and p38 pathways function to modulate cell-cycle, apoptotic and transcriptional responses to stress.
• Uniprot ID: P45983
• Molecular Weight: 50