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Anti-IRS-1 Antibody (Phospho-Ser 312) (OAAI00339)

100 ug
$355.00
In Stock
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Predicted Species Reactivity:
Human, Rat, Mouse
Clonality:
Polyclonal
Isotype:
Rabbit IgG
Host:
Rabbit
Application:
WB, IP
Reconstitution and Storage:
The antibodies are stable for 12 months from date of receipt when stored at -20C to -70C. The antibodies can be stored at 2C to 8C for three month without detectable loss of activity.
Avoid repeated freezing thawing cycles.
Replacement Item:
This antibody may replace item sc-177402 from Santa Cruz Biotechnology.
Immunogen:
A synthetic peptide corresponding to the epitope ATSPA with a single phosphorylation site Ser 312 of Human IRS-1.
Purification:
The Rabbit IgG is purified by Site-specific Epitope Affinity Purification.
Datasheets/Manuals:
Printable datasheet for Rabbit Anti-IRS-1 Antibody (Phospho-Ser 312)OAAI00339
Specificity:
This antibody recognizes ~165 kDa of Human IRS-1 (pSer312). It also reacts with Mouse and Rat. The other species are not tested.
Application Info:
Positive Control: CHO Cell Lysate
Western Blot: 0.1-1 μg/ml
ELISA: 0.01-0.1 μg/ml
IP: 2-5 μg/ml
IHC: 0.5-2 μg/ml
Target Reference:
Li, Y. et al. (2004) J. Biol. Chem. 279, 45304–45307
Gene Symbol:
IRS1
Official Gene Full Name:
insulin receptor substrate 1
Alias Symbols:
HIRS-1,
NCBI Gene Id:
3667
Description of Target:
Insulin Receptor SubstRate-1 (IRS-1), 165 kDa cytoplasmic docking protein, is one of the major endogenous substRates of the insulin receptor kinase. IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2 domain containing proteins, which mediate the metabolic and growth promoting functions of insulin. IRS-1 also contains over 30 potential serine/threonine phosphorylation sites. Ser312 of IRS-1 is phosphorylated by JNK and IKK and Ser789 is phosphorylated by SIK-2, a member of AMPK family. The phosphorylation of Tyr612 and Ser636/639 is mediated by the PKC and mTOR pathways, respectively and phosphorylation at Ser1101 is mediated by PKC, resulting in an inhibition of insulin signaling in the cell, sμggesting a potential mechanism for insulin resistance in some models of obesity.
Swissprot Id:
P35568
Molecular Weight:
165 kDa
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express IRS1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express IRS1.

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